验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, THP-1 cells, U-251 cells |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14014-1-AP targets NFKBIZ in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | NFKBIZ fusion protein Ag5120 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, zeta |
| Synonyms | IKBZ, IkB zeta, I-kappa-B-zeta, IkappaBzeta, IkappaB zeta |
| Calculated Molecular Weight | 78 kDa |
| Observed Molecular Weight | 78 kDa, 64 kDa |
| GenBank Accession Number | BC060800 |
| Gene Symbol | NFKBIZ |
| Gene ID (NCBI) | 64332 |
| RRID | AB_2878000 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BYH8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NFKBIZ, also named as NF-kappa-B inhibitor zeta, is a 718 amino acid protein, which contains 7 ANK repeats and is expressed at high levels in peripheral blood leukocytes and lung, at moderate levels in liver, placenta, and at low levels in spleen, kidney, skeletal muscle and heart. NFKBIZ exists as three isoforms and molecular weight of isoform three is a 64 kDa. The isoform one is 78 kDa protein and isoform two is a 68 kDa protein. NFKBIZ is involved in regulation of NF-kappa-B transcription factor complexes. It Inhibits NF-kappa-B activity without affecting its nuclear translocation upon stimulation.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NFKBIZ antibody 14014-1-AP | Download protocol |
| IF protocol for NFKBIZ antibody 14014-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Immunol Single-Cell Transcriptome Analysis of Chronic Antibody-Mediated Rejection After Renal Transplantation. | ||
Front Mol Neurosci Silencing NUDT21 Attenuates the Mesenchymal Identity of Glioblastoma Cells via the NF-κB Pathway. | ||
Toxicol Appl Pharmacol Tetrachloro-p-benzoquinone induces hepatic oxidative damage and inflammatory response, but not apoptosis in mouse: The prevention of curcumin. | ||
Int J Biol Macromol Charaterization and immunomodulatory activities of polysaccharide isolated from Pleurotus eryngii. | ||
JCI Insight The negative feedback loop of NF-κB/miR-376b/NFKBIZ in septic acute kidney injury.
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