• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

NFX1 Recombinant antibody

NFX1 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230211D9

Cat no : 82924-1-RR

Print datasheet

Synonyms

Transcriptional repressor NF-X1, Nuclear transcription factor, X box-binding protein 1, NFX2, EC:2.3.2.-

验证数据展示

  • FC experiment of PC-3 using 82924-1-RR

    FC experiment of PC-3 using 82924-1-RR

    1x10^6 PC-3 cells were intracellularly stained with 0.25 ug NFX1 Recombinant antibody (82924-1-RR, Clone:230211D9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • IF Staining of HeLa using 82924-1-RR

    IF Staining of HeLa using 82924-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NFX1 antibody (82924-1-RR, Clone: 230211D9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inPC-3 cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82924-1-RR targets NFX1 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen NFX1 fusion protein Ag9448 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name nuclear transcription factor, X-box binding 1
Synonyms Transcriptional repressor NF-X1, Nuclear transcription factor, X box-binding protein 1, NFX2, EC:2.3.2.-
Calculated Molecular Weight 1120 aa, 124 kDa
GenBank Accession NumberBC012151
Gene Symbol NFX1
Gene ID (NCBI) 4799
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ12986
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for NFX1 antibody 82924-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols