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NFX1 Recombinant antibody

NFX1 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

230211H4

Cat no : 82924-2-RR

Print datasheet

Synonyms

DKFZp779G2416, NFX1, NFX2

验证数据展示

  • WB analysis using 82924-2-RR

    WB analysis using 82924-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82924-2-RR (NFX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 82924-2-RR

    Affinity and Kinetic Characterization of 82924-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82924-2-RR against Human NFX1 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHeLa cells, HEK-293T cells, Jurkat cells, K-562cells, HCT 116 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82924-2-RR targets NFX1 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen NFX1 fusion protein Ag9448 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name nuclear transcription factor, X-box binding 1
Synonyms DKFZp779G2416, NFX1, NFX2
Calculated Molecular Weight 1120 aa, 124 kDa
Observed Molecular Weight 124 kDa
GenBank Accession NumberBC012151
Gene Symbol NFX1
Gene ID (NCBI) 4799
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ12986
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NFX1 antibody 82924-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols