验证数据展示
经过测试的应用
Positive WB detected in | K-562 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
产品信息
10129-2-AP targets NFYC in WB, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, ELISA Application Description |
文献引用应用 | WB |
经测试反应性 | human |
文献引用反应性 | mouse |
免疫原 | NFYC fusion protein Ag0140 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | nuclear transcription factor Y, gamma |
别名 | CBF C, CBFC, H1TF2A, HAP5, HSM, NF YC, NFYC, Transactivator HSM 1/2 |
计算分子量 | 37 kDa, 50 kDa |
观测分子量 | 48 kDa |
GenBank蛋白编号 | BC005003 |
基因名称 | NFYC |
Gene ID (NCBI) | 4802 |
RRID | AB_2152244 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q13952 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NFYC, also named as Nuclear transcription factor Y subunit gamma or Transactivator HSM-1/2, is a 458 amino acid protein, which belongs to the NFYC/HAP5 subunit family. NFYC localizes in the nucleus and stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes. SP1, NFYC and FOXO3, FOXO4 transcription factors are involved in the regulation of STK11 transcription.
实验方案
Product Specific Protocols | |
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WB protocol for NFYC antibody 10129-2-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |