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验证数据展示

  • WB analysis of K-562 using 10129-2-AP

    WB analysis of K-562 using 10129-2-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 10129-2-AP (NFYC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

NFYC Polyclonal antibody

NFYC Polyclonal Antibody for WB, ELISA
Cat No. 10129-2-AP

Print datasheet

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, ELISA

Synonyms

CBF C, CBFC, H1TF2A, HAP5, HSM, NF YC, NFYC, Transactivator HSM 1/2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

10129-2-AP targets NFYC in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivitymouse
Immunogen NFYC fusion protein Ag0140 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nuclear transcription factor Y, gamma
Synonyms CBF C, CBFC, H1TF2A, HAP5, HSM, NF YC, NFYC, Transactivator HSM 1/2
Calculated Molecular Weight 37 kDa, 50 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC005003
Gene Symbol NFYC
Gene ID (NCBI) 4802
RRIDAB_2152244
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13952
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NFYC, also named as Nuclear transcription factor Y subunit gamma or Transactivator HSM-1/2, is a 458 amino acid protein, which belongs to the NFYC/HAP5 subunit family. NFYC localizes in the nucleus and stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes. SP1, NFYC and FOXO3, FOXO4 transcription factors are involved in the regulation of STK11 transcription.

实验方案

Product Specific Protocols
WB protocol for NFYC antibody 10129-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Res

Pancreas-specific Pten deficiency causes partial resistance to diabetes and elevated hepatic AKT signaling.

Authors - Tong Zan Z
View Article