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验证数据展示

  • WB analysis using 26991-1-AP (same clone as 26991-1-PBS)

    WB analysis using 26991-1-AP (same clone as 26991-1-PBS)

    HT-29 cells were subjected to SDS PAGE followed by western blot with 26991-1-AP (Lipocalin-2/NGAL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26991-1-PBS in a different storage buffer formulation.

  • WB analysis of HT-29 using 26991-1-AP (same clone as 26991-1-PBS)

    WB analysis of HT-29 using 26991-1-AP (same clone as 26991-1-PBS)

    WB result of NGAL antibody (26991-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NGAL transfected HT-29 cells. This data was developed using the same antibody clone with 26991-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 26991-1-AP (same clone as 26991-1-PBS)

    IF Staining of A431 using 26991-1-AP (same clone as 26991-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using Lipocalin-2/NGAL antibody (26991-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26991-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 26991-1-AP (same clone as 26991-1-PBS)

    IHC staining of human colon cancer using 26991-1-AP (same clone as 26991-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26991-1-AP (Lipocalin-2/NGAL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26991-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NGAL/LCN2 antibody included
  • IHC staining of human colon cancer using 26991-1-AP (same clone as 26991-1-PBS)

    IHC staining of human colon cancer using 26991-1-AP (same clone as 26991-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26991-1-AP (LCN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26991-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NGAL/LCN2 antibody included
  • FC experiment of A431 using 26991-1-AP (same clone as 26991-1-PBS)

    FC experiment of A431 using 26991-1-AP (same clone as 26991-1-PBS)

    1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human Lipocalin-2/NGAL (26991-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 26991-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

Lipocalin-2/NGAL Polyclonal antibody, PBS Only

Lipocalin-2/NGAL Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 26991-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

lipocalin 2, LCN2, NGAL, Lcn 2, HNL

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy NGAL/LCN2 Ready-To-Use IHC Kit. NGAL/LCN2 primary antibody included.

产品信息

26991-1-PBS targets Lipocalin-2/NGAL in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 Lipocalin-2/NGAL fusion protein Ag25715 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 lipocalin 2
别名 lipocalin 2, LCN2, NGAL, Lcn 2, HNL
观测分子量 25 kDa
基因名称 NGAL/LCN2
Gene ID (NCBI) 3934
RRIDAB_2880715
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Lipocalin-2 (LCN-2) is a adipocytokine also referred to as neutrophil gelatinase-associated lipocalin (NGAL). Lipocalin-2 is a circulatory protein responsible for the transportation of small and hydrophobic molecules to target organs. Lipocalin-2 is used as a biomarker for acute and chronic renal injury. It is present in a large variety of cells including neutrophil, hepatocytes, lung, bone marrow, adipose tissue, macrophages, thymus, non-neoplastic breast duct, prostate, and renal cells. Different functions have been associated with Lipocalin-2, including antibacterial, anti-inflammatory, and protection against cell and tissue stress (PMID:34463264).