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验证数据展示

  • WB analysis of human cerebellum using 16524-1-AP

    WB analysis of human cerebellum using 16524-1-AP

    human cerebellum tissue were subjected to SDS PAGE followed by western blot with 16524-1-AP (NGDN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human epididymis using 16524-1-AP

    IHC staining of human epididymis using 16524-1-AP

    Immunohistochemical analysis of paraffin-embedded human epididymis using 16524-1-AP (NGDN antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human epididymis using 16524-1-AP

    IHC staining of human epididymis using 16524-1-AP

    Immunohistochemical analysis of paraffin-embedded human epididymis using 16524-1-AP (NGDN antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 16524-1-AP

    IF Staining of HeLa using 16524-1-AP

    Immunofluorescent analysis of Hela cells, using NGDN antibody 16524-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

NGDN Polyclonal antibody

NGDN Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 16524-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

LCP5, EIF4E-binding protein, EIF4E binding protein, Centromere accumulated nuclear protein 1, CANu1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman cerebellum tissue, mouse brain tissue, mouse testis tissue
Positive IHC detected inhuman epididymis tissue, human heart tissue, human kidney tissue, human liver tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

16524-1-AP targets NGDN in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
免疫原 NGDN fusion protein Ag9763 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 neuroguidin, EIF4E binding protein
别名 LCP5, EIF4E-binding protein, EIF4E binding protein, Centromere accumulated nuclear protein 1, CANu1
计算分子量 315 aa, 36 kDa
观测分子量 36 kDa
GenBank蛋白编号BC030817
基因名称 NGDN
Gene ID (NCBI) 25983
RRIDAB_2152270
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NEJ9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NGDN antibody 16524-1-APDownload protocol
IHC protocol for NGDN antibody 16524-1-APDownload protocol
IF protocol for NGDN antibody 16524-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Nature

A viral Sm-class RNA base-pairs with mRNAs and recruits microRNAs to inhibit apoptosis.

Authors - Carlos Gorbea
View Article