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NGDN Polyclonal antibody

NGDN Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16524-1-AP

Print datasheet

Synonyms

LCP5, EIF4E-binding protein, EIF4E binding protein, Centromere accumulated nuclear protein 1, CANu1

验证数据展示

  • WB analysis of human cerebellum using 16524-1-AP

    WB analysis of human cerebellum using 16524-1-AP

    human cerebellum tissue were subjected to SDS PAGE followed by western blot with 16524-1-AP (NGDN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human epididymis using 16524-1-AP

    IHC staining of human epididymis using 16524-1-AP

    Immunohistochemical analysis of paraffin-embedded human epididymis using 16524-1-AP (NGDN antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human epididymis using 16524-1-AP

    IHC staining of human epididymis using 16524-1-AP

    Immunohistochemical analysis of paraffin-embedded human epididymis using 16524-1-AP (NGDN antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 16524-1-AP

    IF Staining of HeLa using 16524-1-AP

    Immunofluorescent analysis of Hela cells, using NGDN antibody 16524-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

经过测试的应用

Positive WB detected inhuman cerebellum tissue, mouse brain tissue, mouse testis tissue
Positive IHC detected inhuman epididymis tissue, human heart tissue, human kidney tissue, human liver tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

16524-1-AP targets NGDN in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Immunogen NGDN fusion protein Ag9763 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name neuroguidin, EIF4E binding protein
Synonyms LCP5, EIF4E-binding protein, EIF4E binding protein, Centromere accumulated nuclear protein 1, CANu1
Calculated Molecular Weight 315 aa, 36 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC030817
Gene Symbol NGDN
Gene ID (NCBI) 25983
RRIDAB_2152270
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NEJ9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NGDN antibody 16524-1-APDownload protocol
IHC protocol for NGDN antibody 16524-1-APDownload protocol
IF protocol for NGDN antibody 16524-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Nature

A viral Sm-class RNA base-pairs with mRNAs and recruits microRNAs to inhibit apoptosis.

Authors - Carlos Gorbea
View Article