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验证数据展示

  • WB analysis using 13271-1-AP

    WB analysis using 13271-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13271-1-AP (NGEF antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 13271-1-AP

    IHC staining of human brain using 13271-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13271-1-AP (NGEF antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NGEF antibody included
  • IHC staining of human brain using 13271-1-AP

    IHC staining of human brain using 13271-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13271-1-AP (NGEF antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NGEF antibody included
  • IP experiment of mouse brain using 13271-1-AP

    IP experiment of mouse brain using 13271-1-AP

    IP result of anti-NGEF (IP:13271-1-AP, 4ug; Detection:13271-1-AP 1:500) with mouse brain tissue lysate 4000ug.

NGEF Polyclonal antibody

NGEF Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 13271-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA

别名

EPHEXIN, Ephexin 1, Ephexin-1, NGEF

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, mouse testis tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy NGEF Ready-To-Use IHC Kit. NGEF primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

13271-1-AP targets NGEF in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性rat
免疫原 NGEF fusion protein Ag3967 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 neuronal guanine nucleotide exchange factor
别名 EPHEXIN, Ephexin 1, Ephexin-1, NGEF
计算分子量 710 aa, 82 kDa
观测分子量 71 kDa
GenBank蛋白编号BC031573
基因名称 NGEF
Gene ID (NCBI) 25791
RRIDAB_2152381
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N5V2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NGEF antibody 13271-1-APDownload protocol
IHC protocol for NGEF antibody 13271-1-APDownload protocol
IP protocol for NGEF antibody 13271-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Brain Res Bull

Electroacupuncture ameliorates chronic unpredictable mild stress-induced depression-like behavior and cognitive impairment through suppressing oxidative stress and neuroinflammation in rats

Authors - Tao Tong
View Article