• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

NGEF Polyclonal antibody

NGEF Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13271-1-AP

Print datasheet

Synonyms

EPHEXIN, Ephexin 1, Ephexin-1, NGEF

验证数据展示

  • WB analysis using 13271-1-AP

    WB analysis using 13271-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13271-1-AP (NGEF antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 13271-1-AP

    IHC staining of human brain using 13271-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13271-1-AP (NGEF antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human brain using 13271-1-AP

    IHC staining of human brain using 13271-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13271-1-AP (NGEF antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of mouse brain using 13271-1-AP

    IP experiment of mouse brain using 13271-1-AP

    IP result of anti-NGEF (IP:13271-1-AP, 4ug; Detection:13271-1-AP 1:500) with mouse brain tissue lysate 4000ug.

经过测试的应用

Positive WB detected inmouse brain tissue, mouse testis tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

13271-1-AP targets NGEF in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityrat
Immunogen NGEF fusion protein Ag3967 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name neuronal guanine nucleotide exchange factor
Synonyms EPHEXIN, Ephexin 1, Ephexin-1, NGEF
Calculated Molecular Weight 710 aa, 82 kDa
Observed Molecular Weight 71 kDa
GenBank Accession NumberBC031573
Gene Symbol NGEF
Gene ID (NCBI) 25791
RRIDAB_2152381
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N5V2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NGEF antibody 13271-1-APDownload protocol
IHC protocol for NGEF antibody 13271-1-APDownload protocol
IP protocol for NGEF antibody 13271-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Brain Res Bull

Electroacupuncture ameliorates chronic unpredictable mild stress-induced depression-like behavior and cognitive impairment through suppressing oxidative stress and neuroinflammation in rats

Authors - Tao Tong
View Article