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NHE1 Polyclonal antibody

NHE1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat No : 29761-1-AP

Print datasheet

Synonyms

APNH, APNH1, FLJ42224, Na(+)/H(+) exchanger 1, NHE 1, NHE1, SLC9A1, Sodium/hydrogen exchanger 1

验证数据展示

  • WB analysis using 29761-1-AP

    WB analysis using 29761-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29761-1-AP (NHE1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 29761-1-AP

    WB analysis using 29761-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29761-1-AP (NHE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 29761-1-AP

    IHC staining of human stomach using 29761-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 29761-1-AP (NHE1 antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with NHE1/SLC9A1 antibody included
  • IHC staining of human stomach using 29761-1-AP

    IHC staining of human stomach using 29761-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 29761-1-AP (NHE1 antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with NHE1/SLC9A1 antibody included

经过测试的应用

Positive WB detected inmouse brain tissue, mouse heart tissue, rat brain tissue, rat heart tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy NHE1/SLC9A1 Ready-To-Use IHC Kit. NHE1/SLC9A1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29761-1-AP targets NHE1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen NHE1 fusion protein Ag31192 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 9 (sodium/hydrogen exchanger), member 1
Synonyms APNH, APNH1, FLJ42224, Na(+)/H(+) exchanger 1, NHE 1, NHE1, SLC9A1, Sodium/hydrogen exchanger 1
Calculated Molecular Weight 91 kDa
Observed Molecular Weight90-100 kDa
GenBank Accession NumberNM_003047
Gene Symbol NHE1
Gene ID (NCBI) 6548
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP19634
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NHE1, also named as SLC9A1, encodes an Na+/H+ antiporter that is a membrane-bound enzyme involved in regulating pH of vertebrate cells. NHE1 regulates a number of cell behaviors, including adhesion, shape determination, migration, and proliferation (PMID:11807182). It is inhibited by the non-specific diuretic drug amiloride and activated by growth factors, mitogens, neurotransmitters, tumor promoters, and others (PMID: 2846238). NHE1 is expressed in heart where it contributes to cardiomyocyte pH homeostasis (PMID: 12832126, PMID: 23709596).

实验方案

Product Specific Protocols
WB protocol for NHE1 antibody 29761-1-APDownload protocol
IHC protocol for NHE1 antibody 29761-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols