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  • Featured Product
  • KD/KO Validated

NHE1 Monoclonal antibody, PBS Only

NHE1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3D9F3

Cat no : 67363-1-PBS

Print datasheet

Synonyms

APNH, APNH1, FLJ42224, Na(+)/H(+) exchanger 1, NHE 1, NHE1, SLC9A1, Sodium/hydrogen exchanger 1

验证数据展示

  • WB analysis using 67363-1-Ig (same clone as 67363-1-PBS)

    WB analysis using 67363-1-Ig (same clone as 67363-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67363-1-Ig (NHE1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 67363-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 67363-1-Ig (same clone as 67363-1-PBS)

    IHC staining of human liver using 67363-1-Ig (same clone as 67363-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67363-1-Ig (NHE1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67363-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NHE1/SLC9A1 antibody included
Planning an IHC experiment? We recommend our IHCeasy NHE1/SLC9A1 Ready-To-Use IHC Kit. NHE1/SLC9A1 primary antibody included.

产品信息

67363-1-PBS targets NHE1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity Human, Rat
Immunogen NHE1 fusion protein Ag14329 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name solute carrier family 9 (sodium/hydrogen exchanger), member 1
Synonyms APNH, APNH1, FLJ42224, Na(+)/H(+) exchanger 1, NHE 1, NHE1, SLC9A1, Sodium/hydrogen exchanger 1
Calculated Molecular Weight 815 aa, 91 kDa
Observed Molecular Weight 110 kDa
GenBank Accession NumberBC012121
Gene Symbol NHE1
Gene ID (NCBI) 6548
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP19634
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NHE1, also named as SLC9A1, is involved in pH regulation that can eliminate acids generated by active metabolism and counter adverse environmental conditions. NHE1 plays an important role in signal transduction. It has been shown that NHE1 participates in the development and progression of human breast cancer, gastric cancer and others. NHE1 has some isoforms with 91 kDa , 110 kDa (phosphoglycoprotein) and 210 kDa (dimeric protein). (PMID:28098891, 27751915, 8300588)