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验证数据展示

  • IHC staining of human brain using 24065-1-AP

    IHC staining of human brain using 24065-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24065-1-AP (NHEDC2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human brain using 24065-1-AP

    IHC staining of human brain using 24065-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24065-1-AP (NHEDC2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of human plasma using 24065-1-AP

    WB analysis of human plasma using 24065-1-AP

    human plasma were subjected to SDS PAGE followed by western blot with 24065-1-AP (NHEDC2 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

NHEDC2 Polyclonal antibody

NHEDC2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24065-1-AP

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

NHA2, NHE10, NHEDC2, SLC9B2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inhuman plasma tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24065-1-AP targets NHEDC2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen NHEDC2 fusion protein Ag21141 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Na+/H+ exchanger domain containing 2
Synonyms NHA2, NHE10, NHEDC2, SLC9B2
Calculated Molecular Weight 537 aa, 58 kDa
Observed Molecular Weight ~55 kDa
GenBank Accession NumberBC047447
Gene Symbol NHEDC2
Gene ID (NCBI) 133308
RRIDAB_2879423
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ86UD5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NHEDC2, also named as NHA2 and SLC9B2, contributes to the organellar volume homeostasis. It is required for osteoclast differentiation and bone resorption activity. NHEDC2 has two isoforms with MW 48 kDa and 58 kDa.

实验方案

Product Specific Protocols
WB protocol for NHEDC2 antibody 24065-1-APDownload protocol
IHC protocol for NHEDC2 antibody 24065-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols