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验证数据展示

  • WB analysis of L02 using 21310-1-AP

    WB analysis of L02 using 21310-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 21310-1-AP (NHLRC1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 21310-1-AP

    IHC staining of human heart using 21310-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 21310-1-AP (NHLRC1 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 21310-1-AP

    IHC staining of human heart using 21310-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 21310-1-AP (NHLRC1 antibody) at dilution of 1:50 (under 40x lens).

NHLRC1 Polyclonal antibody

NHLRC1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21310-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

EPM2B, MALIN, NHL repeat containing 1, NHLRC1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inL02 cells
Positive IHC detected inhuman heart tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21310-1-AP targets NHLRC1 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 NHLRC1 fusion protein Ag15940 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NHL repeat containing 1
别名 EPM2B, MALIN, NHL repeat containing 1, NHLRC1
计算分子量 395 aa, 42 kDa
观测分子量 45-47 kDa
GenBank蛋白编号BC103888
基因名称 NHLRC1
Gene ID (NCBI) 378884
RRIDAB_10733882
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6VVB1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NHLRC1(NHL repeat-containing protein 1) is also named as Malin,EPM2B. It is predicted to encode a 42.3-kDa (395amino acid) protein, malin, containing a detectable zinc-binding RING finger and six NHL-repeat domains(PMID:12958597).It can suppresses the cellular toxicity of misfolded proteins by promoting their degradation through the ubiquitin-proteasome system (UPS).Defects in NHLRC1 are a cause of progressive myoclonic epilepsy type 2 (EPM2).This antibody is specific to NHLRC1.

实验方案

Product Specific Protocols
WB protocol for NHLRC1 antibody 21310-1-APDownload protocol
IHC protocol for NHLRC1 antibody 21310-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols