验证数据展示
经过测试的应用
| Positive WB detected in | SH-SY5Y cells, Caco-2 cells, HeLa cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15128-1-AP targets NHP2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IHC, IF, IP, CoIP |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, yeast |
| Immunogen | NHP2 fusion protein Ag7251 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | NHP2 ribonucleoprotein homolog (yeast) |
| Synonyms | NOLA2, NHP2P, NHP 2, HSPC286 |
| Calculated Molecular Weight | 17 kDa |
| Observed Molecular Weight | 17-20 kDa |
| GenBank Accession Number | BC000009 |
| Gene Symbol | NHP2 |
| Gene ID (NCBI) | 55651 |
| RRID | AB_2267290 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NX24 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NHP2 belongs to the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. It is required for ribosome biogenesis and telomere maintenance, and for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NHP2 antibody 15128-1-AP | Download protocol |
| IHC protocol for NHP2 antibody 15128-1-AP | Download protocol |
| IF protocol for NHP2 antibody 15128-1-AP | Download protocol |
| IP protocol for NHP2 antibody 15128-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
EMBO J NHP2 downregulation counteracts hTR-mediated activation of the DNA damage response at ALT telomeres.
| ||
EMBO Rep Nhp2 is a reader of H2AQ105me and part of a network integrating metabolism with rRNA synthesis. | ||
Mol Cell Biol SUMOylation- and GAR1-Dependent Regulation of Dyskerin Nuclear and Subnuclear Localization. |
