验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, MCF-7 cells, human brain tissue, mouse pancreas tissue, HepG2 cells, Jurkat cells, SH-SY5Y cells, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | human cervical cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
Planning an IHC experiment? We recommend our IHCeasy SNU13/NHP2L1 Ready-To-Use IHC Kit. SNU13/NHP2L1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15802-1-AP targets NHP2L1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | NHP2L1 fusion protein Ag8532 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | NHP2 non-histone chromosome protein 2-like 1 (S. cerevisiae) |
| 别名 | SNU13, OTK27, NHP2-like protein 1, N-terminally processed, NHP2-like protein 1, hSNU13 |
| 计算分子量 | 128 aa, 14 kDa |
| 观测分子量 | 14 kDa |
| GenBank蛋白编号 | BC005358 |
| 基因名称 | NHP2L1 |
| Gene ID (NCBI) | 4809 |
| RRID | AB_2251452 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P55769 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NHP2L1, belongs to the ribosomal protein L7Ae family, is a component of the spliceosome complex. It is recruited to introns following the attachment of U4 and U6 RNAs, and is required for subsequent recruitment of PRP31 and activation of the spliceosomal complex [PMID: 17412961]. It binds to the 5'-stem-loop of U4 snRNA and may have a role in the late stage of spliceosome assembly. The protein undergoes a conformational change upon RNA-binding [PMID: 10545122].
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NHP2L1 antibody 15802-1-AP | Download protocol |
| IHC protocol for NHP2L1 antibody 15802-1-AP | Download protocol |
| IF protocol for NHP2L1 antibody 15802-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell Nascent Pre-rRNA Sorting via Phase Separation Drives the Assembly of Dense Fibrillar Components in the Human Nucleolus.
| ||
Mol Cell Condensates induced by transcription inhibition localize active chromatin to nucleoli. | ||
Mol Cell LARP7-Mediated U6 snRNA Modification Ensures Splicing Fidelity and Spermatogenesis in Mice. | ||
Biochim Biophys Acta Proteins Proteom Phosphoproteomic response of cardiac endothelial cells to ischemia and ultrasound. |
