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Nidogen 2 Polyclonal antibody

Nidogen 2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19076-1-AP

Print datasheet

Synonyms

NID 2, NID2, Nidogen 2, nidogen 2 (osteonidogen), Osteonidogen

验证数据展示

  • WB analysis of human placenta using 19076-1-AP

    WB analysis of human placenta using 19076-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 19076-1-AP (Nidogen 2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 19076-1-AP

    IHC staining of human placenta using 19076-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 19076-1-AP (Nidogen 2 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human placenta using 19076-1-AP

    IHC staining of human placenta using 19076-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 19076-1-AP (Nidogen 2 antibody) at dilution of 1:100 (under 40x lens).

经过测试的应用

Positive WB detected inhuman placenta tissue, mouse heart tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19076-1-AP targets Nidogen 2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Nidogen 2 fusion protein Ag4643 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nidogen 2 (osteonidogen)
Synonyms NID 2, NID2, Nidogen 2, nidogen 2 (osteonidogen), Osteonidogen
Calculated Molecular Weight 1375 aa, 151 kDa
Observed Molecular Weight 200 kDa
GenBank Accession NumberBC035608
Gene Symbol NID2
Gene ID (NCBI) 22795
RRIDAB_2153428
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14112
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Nidogen 2 antibody 19076-1-APDownload protocol
IHC protocol for Nidogen 2 antibody 19076-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols