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验证数据展示

  • WB analysis using 13007-1-AP

    WB analysis using 13007-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13007-1-AP (Ninein antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 13007-1-AP

    IHC staining of mouse brain using 13007-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13007-1-AP (Ninein antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 13007-1-AP

    IHC staining of mouse brain using 13007-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13007-1-AP (Ninein antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 13007-1-AP

    IF Staining of HeLa using 13007-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Ninein antibody (13007-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of MCF-7 using 13007-1-AP

    IF Staining of MCF-7 using 13007-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Ninein antibody (13007-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

Ninein Polyclonal antibody

Ninein Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 13007-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

NIN, KIAA1565, hNinein, GSK3B-interacting protein, GSK3B interacting protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, MCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13007-1-AP targets Ninein in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原 Ninein fusion protein Ag3688 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ninein (GSK3B interacting protein)
别名 NIN, KIAA1565, hNinein, GSK3B-interacting protein, GSK3B interacting protein
计算分子量 2090 aa, 243 kDa
观测分子量 240-255 kDa
GenBank蛋白编号BC034708
基因名称 Ninein
Gene ID (NCBI) 51199
RRIDAB_3085405
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N4C6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ninein is a large coiled-coil protein that associates with the subdistal appendages of the mother centriole and the minus-ends of both centrioles. Ninein interacts with γ-tubulin and microtubule nucleation and anchoring at the centrosomes.

实验方案

Product Specific Protocols
WB protocol for Ninein antibody 13007-1-APDownload protocol
IHC protocol for Ninein antibody 13007-1-APDownload protocol
IF protocol for Ninein antibody 13007-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

EMBO J

Multimodal mechanisms of human centriole engagement and disengagement

Authors - Kei K Ito
View Article
humanWB,IF

Dev Cell

Comparative Super-Resolution Mapping of Basal Feet Reveals a Modular but Distinct Architecture in Primary and Motile Cilia.

Authors - Quynh P H Nguyen
  • KD Validated
View Article
mouseIF

Dev Dyn

Fused (Stk36) is a ciliary protein required for central pair assembly and motile cilia orientation in the mammalian oviduct.

Authors - Nozawa Yoko Inès YI
View Article