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验证数据展示

  • WB analysis of HeLa using 16830-1-AP

    WB analysis of HeLa using 16830-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16830-1-AP (NIP30 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 16830-1-AP

    IP experiment of HeLa using 16830-1-AP

    IP result of anti-NIP30 (IP:16830-1-AP, 4ug; Detection:16830-1-AP 1:500) with HeLa cells lysate 1080ug.

  • IF Staining of HeLa using 16830-1-AP

    IF Staining of HeLa using 16830-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 16830-1-AP (NIP30 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

NIP30 Polyclonal antibody

NIP30 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 16830-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IP, ELISA

别名

PA28G-interacting protein, NEFA-interacting nuclear protein NIP30, FAM192A, CDA10, CDA018

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

16830-1-AP targets NIP30 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 NIP30 fusion protein Ag10439 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NEFA-interacting nuclear protein NIP30
别名 PA28G-interacting protein, NEFA-interacting nuclear protein NIP30, FAM192A, CDA10, CDA018
计算分子量 254 aa, 29 kDa
观测分子量 29 kDa
GenBank蛋白编号BC071952
基因名称 NIP30
Gene ID (NCBI) 80011
RRIDAB_2109703
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9GZU8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NIP30, also known as NEFA-interacting nuclear protein 30, FAM192A (family with sequence similarity 192, member A), is a 254 amino acid nuclear protein encoded by a gene that maps to human chromosome 16q13.

实验方案

Product Specific Protocols
WB protocol for NIP30 antibody 16830-1-APDownload protocol
IF protocol for NIP30 antibody 16830-1-APDownload protocol
IP protocol for NIP30 antibody 16830-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

The REGγ inhibitor NIP30 increases sensitivity to chemotherapy in p53-deficient tumor cells.

Authors - Xiao Gao
View Article
humanWB

Retrovirology

Comparative host protein interactions with HTLV-1 p30 and HTLV-2 p28: insights into difference in pathobiology of human retroviruses.

Authors - Doueiri Rami R
View Article