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验证数据展示

  • WB analysis using 16839-1-AP

    WB analysis using 16839-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16839-1-AP (NIP7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 16839-1-AP

    IP experiment of HeLa using 16839-1-AP

    IP result of anti-NIP7 (IP:16839-1-AP, 4ug; Detection:16839-1-AP 1:1000) with HeLa cells lysate 1600 ug.

NIP7 Polyclonal antibody

NIP7 Polyclonal Antibody for WB, IP, ELISA
Cat No. 16839-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA

别名

CGI 37, HSPC031, HSPC180, KD93, NIP7, OK/SW cl.76, OK/SW cl.78

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IP detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16839-1-AP targets NIP7 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 NIP7 fusion protein Ag10526 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nuclear import 7 homolog (S. cerevisiae)
别名 CGI 37, HSPC031, HSPC180, KD93, NIP7, OK/SW cl.76, OK/SW cl.78
计算分子量 180 aa, 20 kDa
观测分子量 20-22 kDa
GenBank蛋白编号BC015941
基因名称 NIP7
Gene ID (NCBI) 51388
RRIDAB_2153549
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y221
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nip7 was initially identified in yeast as required for processing of the 27S pre-rRNA to form the mature 25S and 5.8S rRNAs (PMID: 9891085). It localizes to the nucleolus but was also found to sediment in the region of free 60S subunits in sucrose density gradients (PMID: 9891085). Experimental evidence suggests that the P. abyssi Nip7 may be an exosome regulatory factor. It binds preferentially to U- and AU-rich RNAs and strongly inhibits the exosome due to its association with both the exosome complex and the substrate RNA.

实验方案

Product Specific Protocols
WB protocol for NIP7 antibody 16839-1-APDownload protocol
IP protocol for NIP7 antibody 16839-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols