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NIP7 Polyclonal antibody

NIP7 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 16839-1-AP

Print datasheet

Synonyms

CGI 37, HSPC031, HSPC180, KD93, NIP7, OK/SW cl.76, OK/SW cl.78

验证数据展示

  • WB analysis using 16839-1-AP

    WB analysis using 16839-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16839-1-AP (NIP7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 16839-1-AP

    IP experiment of HeLa using 16839-1-AP

    IP result of anti-NIP7 (IP:16839-1-AP, 4ug; Detection:16839-1-AP 1:1000) with HeLa cells lysate 1600 ug.

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IP detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16839-1-AP targets NIP7 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen NIP7 fusion protein Ag10526 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nuclear import 7 homolog (S. cerevisiae)
Synonyms CGI 37, HSPC031, HSPC180, KD93, NIP7, OK/SW cl.76, OK/SW cl.78
Calculated Molecular Weight 180 aa, 20 kDa
Observed Molecular Weight 20-22 kDa
GenBank Accession NumberBC015941
Gene Symbol NIP7
Gene ID (NCBI) 51388
RRIDAB_2153549
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y221
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nip7 was initially identified in yeast as required for processing of the 27S pre-rRNA to form the mature 25S and 5.8S rRNAs (PMID: 9891085). It localizes to the nucleolus but was also found to sediment in the region of free 60S subunits in sucrose density gradients (PMID: 9891085). Experimental evidence suggests that the P. abyssi Nip7 may be an exosome regulatory factor. It binds preferentially to U- and AU-rich RNAs and strongly inhibits the exosome due to its association with both the exosome complex and the substrate RNA.

实验方案

Product Specific Protocols
WB protocol for NIP7 antibody 16839-1-APDownload protocol
IP protocol for NIP7 antibody 16839-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols