验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18792-1-AP targets NIPBL in WB, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IP, ChIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | NIPBL fusion protein Ag5663 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Nipped-B homolog (Drosophila) |
| 别名 | CDLS, CDLS1, Delangin, IDN3, IDN3 B, NIPBL, Nipped B homolog (Drosophila), Nipped B like protein, SCC2 homolog |
| 计算分子量 | 2804 aa, 316 kDa |
| 观测分子量 | 300 kDa |
| GenBank蛋白编号 | BC033847 |
| 基因名称 | NIPBL |
| Gene ID (NCBI) | 25836 |
| RRID | AB_2151288 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q6KC79 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NIPBL, also named as Nipped-B-like protein, is a 28-4 amino acid protein, which Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains and belongs to the SCC2/Nipped-B family. NIPBL probably plays a structural role in chromatin and Involve in sister chromatid cohesion, possibly by interacting with the cohesin complex. endogenous full length NIPBL which is estimated to be about 300kDa led us to believe that the NIPBL protein was deteriorated or lost by the standard protein extraction and blotting techniques.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NIPBL antibody 18792-1-AP | Download protocol |
| IF protocol for NIPBL antibody 18792-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun C/EBPβ enhances platinum resistance of ovarian cancer cells by reprogramming H3K79 methylation. | ||
Nat Immunol BRWD1 orchestrates small pre-B cell chromatin topology by converting static to dynamic cohesin | ||
Nucleic Acids Res BRD2 promotes antibody class switch recombination by facilitating DNA repair in collaboration with NIPBL |
