验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18792-1-AP targets NIPBL in WB, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IP, ChIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | NIPBL fusion protein Ag5663 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | Nipped-B homolog (Drosophila) |
| Synonyms | CDLS, CDLS1, Delangin, IDN3, IDN3 B, NIPBL, Nipped B homolog (Drosophila), Nipped B like protein, SCC2 homolog |
| Calculated Molecular Weight | 2804 aa, 316 kDa |
| Observed Molecular Weight | 300 kDa |
| GenBank Accession Number | BC033847 |
| Gene Symbol | NIPBL |
| Gene ID (NCBI) | 25836 |
| RRID | AB_2151288 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6KC79 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NIPBL, also named as Nipped-B-like protein, is a 28-4 amino acid protein, which Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains and belongs to the SCC2/Nipped-B family. NIPBL probably plays a structural role in chromatin and Involve in sister chromatid cohesion, possibly by interacting with the cohesin complex. endogenous full length NIPBL which is estimated to be about 300kDa led us to believe that the NIPBL protein was deteriorated or lost by the standard protein extraction and blotting techniques.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NIPBL antibody 18792-1-AP | Download protocol |
| IF protocol for NIPBL antibody 18792-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun C/EBPβ enhances platinum resistance of ovarian cancer cells by reprogramming H3K79 methylation. | ||
Nat Immunol BRWD1 orchestrates small pre-B cell chromatin topology by converting static to dynamic cohesin | ||
Nucleic Acids Res BRD2 promotes antibody class switch recombination by facilitating DNA repair in collaboration with NIPBL |
