• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 10751-1-AP

    WB analysis using 10751-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10751-1-AP (NIPSNAP3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 10751-1-AP

    IP experiment of mouse brain using 10751-1-AP

    IP result of anti-NIPSNAP3A (IP:10751-1-AP, 4ug; Detection:10751-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

  • IHC staining of human colon cancer using 10751-1-AP

    IHC staining of human colon cancer using 10751-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10751-1-AP (NIPSNAP3A Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human colon cancer using 10751-1-AP

    IHC staining of human colon cancer using 10751-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10751-1-AP (NIPSNAP3A Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of HEK-293 using 10751-1-AP

    IF Staining of HEK-293 using 10751-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using NIPSNAP3A antibody (10751-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

NIPSNAP3A Polyclonal antibody

NIPSNAP3A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 10751-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

TassC, Target for Salmonella secreted protein C, Protein NipSnap homolog 4, Protein NipSnap homolog 3A, NipSnap4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inmouse brain tissue, COLO 320 cells, HEK-293 cells, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10751-1-AP targets NIPSNAP3A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 NIPSNAP3A fusion protein Ag1143 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nipsnap homolog 3A (C. elegans)
别名 TassC, Target for Salmonella secreted protein C, Protein NipSnap homolog 4, Protein NipSnap homolog 3A, NipSnap4
计算分子量 28 kDa
观测分子量 28-33 kDa
GenBank蛋白编号BC005935
基因名称 NIPSNAP3A
Gene ID (NCBI) 25934
RRIDAB_2298420
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UFN0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NIPSNAP3A antibody 10751-1-APDownload protocol
IHC protocol for NIPSNAP3A antibody 10751-1-APDownload protocol
IF protocol for NIPSNAP3A antibody 10751-1-APDownload protocol
IP protocol for NIPSNAP3A antibody 10751-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Gene

NIPSNAP3A regulates cellular homeostasis by modulating mitochondrial dynamics

Authors - Run Yan
  • KD Validated
View Article