验证数据展示
经过测试的应用
| Positive WB detected in | mouse kidney tissue, HEK-293 cells, mouse colon tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16699-1-AP targets NKD2 in WB, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IP, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | NKD2 fusion protein Ag10094 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | naked cuticle homolog 2 (Drosophila) |
| 别名 | Naked-2, Naked2, Naked 2, hNkd2 |
| 计算分子量 | 50 kDa |
| 观测分子量 | 59 kDa |
| GenBank蛋白编号 | BC012176 |
| 基因名称 | NKD2 |
| Gene ID (NCBI) | 85409 |
| RRID | AB_2151468 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q969F2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Naked2 (protein naked cuticle homolog 2), encoded by NKD2 gene, was identified as a cell autonomous antagonist of the canonical Wnt signaling pathway. Naked2 acts as a cargo recognition and targeting protein to ensure proper delivery and fusion of TGF-alpha-containing vesicles to a distinct region at the basolateral surface of polarized epithelial cells (PMID: 17553928). EGFR-independent action of TGF-alpha protects Naked2 from proteasomal degradation, thus ensuring its delivery to the basolateral surface (PMID: 18757723). Myristoylated Naked2 antagonizes Wnt-beta-catenin activity possibly by degrading Dishevelled-1 at the plasma membrane (PMID: 20177058).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NKD2 antibody 16699-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Oncogene NKD2, a negative regulator of Wnt signaling, suppresses tumor growth and metastasis in osteosarcoma.
| ||
Biosci Biotechnol Biochem Silencing of lncRNA ZFAS1 inhibits malignancies by blocking Wnt/β-catenin signaling in gastric cancer cells. | ||
Kaohsiung J Med Sci Curcumin suppresses the Wnt/β-catenin signaling pathway by inhibiting NKD2 methylation to ameliorate intestinal ischemia/reperfusion injury | ||
Rejuvenation Res Tripartite Motif-Containing Protein 65 Promotes Proliferation and Inhibits Ferroptosis in Prostate Cancer via Enhancing NKD Inhibitor of WNT Signaling Pathway 2 Ubiquitination |
