验证数据展示
经过测试的应用
| Positive FC detected in | C57 mouse splenic NK cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.5 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
65100-1-Ig targets CD314/NKG2D in FC applications and shows reactivity with Mouse samples.
| 经测试应用 | FC Application Description |
| 经测试反应性 | Mouse |
| 免疫原 |
Purified Mouse NKG2D protein 种属同源性预测 |
| 宿主/亚型 | Rat / IgG1, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | killer cell lectin-like receptor subfamily K, member 1 |
| 别名 | D6H12S2489E, Klrk1, NKG2 D, Nkg2d, NKG2D (CD314) |
| GenBank蛋白编号 | BC057147 |
| 基因名称 | CD314 |
| Gene ID (NCBI) | 27007 |
| RRID | AB_3084899 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | O54709 |
| 储存缓冲液 | PBS with 0.09% sodium azide, pH 7.3. |
| 储存条件 | Store at 2-8°C. Stable for one year after shipment. |
背景介绍
CD314, also known as NKG2D or Killer cell lectin-like receptor subfamily K member 1 (KLRK1), is a type II lectin-like transmembrane stimulatory receptor (PMID: 8436421). In mice, CD314 is expressed on NK cells, activated CD8(+) T cells and macrophages, and subsets of TCR gamma delta (+) and NK1.1 (+) T cells (PMID: 12150888). Various subfamilies of MHC class I-related glycoproteins have been identified as the ligands for mouse CD314, including RAET1A, RAET1B, RAET1C, RAET1D, RAET1E, H60 and MULT1 (PMID: 31720075).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CD314/NKG2D antibody 65100-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
