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NKX1-2 Polyclonal antibody

NKX1-2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55484-1-AP

Print datasheet

Synonyms

bB238F13.2, C10orf121, Homeobox protein SAX 1, NK1 homeobox 2, NKX 1.1, NKX1 2, NKX1.1, NKX1-2

验证数据展示

  • WB analysis of SH-SY5Y using 55484-1-AP

    WB analysis of SH-SY5Y using 55484-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 55484-1-AP (NKX1-2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 55484-1-AP

    IHC staining of human brain using 55484-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 55484-1-AP (NKX1-2 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inSH-SY5Y cells, mouse brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55484-1-AP targets NKX1-2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name NK1 homeobox 2
Synonyms bB238F13.2, C10orf121, Homeobox protein SAX 1, NK1 homeobox 2, NKX 1.1, NKX1 2, NKX1.1, NKX1-2
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberNM_001146340
Gene Symbol NKX1-2
Gene ID (NCBI) 390010
RRIDAB_2881347
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UD57
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NKX1-2 antibody 55484-1-APDownload protocol
IHC protocol for NKX1-2 antibody 55484-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols