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  • Featured Product
  • KD/KO Validated

NKX2-5 Polyclonal antibody, PBS Only

NKX2-5 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

Cat no : 13921-1-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis of human heart using 13921-1-AP (same clone as 13921-1-PBS)

    WB analysis of human heart using 13921-1-AP (same clone as 13921-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 13921-1-AP (NKX2-5 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13921-1-PBS in a different storage buffer formulation.

  • IHC staining of human brain using 13921-1-AP (same clone as 13921-1-PBS)

    IHC staining of human brain using 13921-1-AP (same clone as 13921-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 13921-1-AP (NKX2-5 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13921-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 13921-1-AP (same clone as 13921-1-PBS)

    IHC staining of mouse heart using 13921-1-AP (same clone as 13921-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13921-1-AP (NKX2-5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13921-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 13921-1-AP (same clone as 13921-1-PBS)

    IHC staining of mouse heart using 13921-1-AP (same clone as 13921-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13921-1-AP (NKX2-5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13921-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse embryo using 13921-1-AP (same clone as 13921-1-PBS)

    IF Staining of mouse embryo using 13921-1-AP (same clone as 13921-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using NKX2-5 antibody (13921-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13921-1-PBS in a different storage buffer formulation.

产品信息

13921-1-PBS targets NKX2-5 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF-P, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen NKX2-5 fusion protein Ag4930 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name NK2 transcription factor related, locus 5 (Drosophila)
Synonyms
Calculated Molecular Weight 324 aa, 35 kDa
Observed Molecular Weight30-42 kda
GenBank Accession NumberBC025711
Gene Symbol NKX2-5
Gene ID (NCBI) 1482
RRIDAB_1851479
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP52952
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Homeobox-containing genes play critical roles in regulating tissue-specific gene expression essential for tissue differentiation, as well as determining the temporal and spatial patterns of development [PMID: 7665173]. NKX2-5 encoded by homeobox-containing genes that implicated in commitment to and/or differentiation of the myocardial lineage, and acts as a transcriptional activator of ANF in cooperation with GATA4. During embryonic development, NKX2-5 plays pivotal roles in specifying cardiac progenitors, cardiac morphogenesis, cardiomyocyte differentiation and conduction system development [PMID: 22824467].