验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20974-1-AP targets NKX6-1 in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | NK6 homeobox 1 |
| 别名 | Homeobox protein Nkx 6.1, NK6 homeobox 1, NKX6 1, NKX6.1, NKX6-1, NKX6A |
| 计算分子量 | 38 kDa |
| 观测分子量 | 45-52 kDa |
| GenBank蛋白编号 | NM_006168 |
| 基因名称 | NKX6-1 |
| Gene ID (NCBI) | 4825 |
| RRID | AB_2918068 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P78426 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NK6 homeobox 1 (NKX6-1) is a potent bifunctional transcription regulator that binds to AT-rich DNA sequences within the promoter region of target genes. NKX6-1 belongs to the class II proteins of neuronal progenitor factors, and the function of it is to enhance proliferation, glucose-stimulated insulin secretion, and survival in mature pancreatic beta-cells, and it is also essential in regional patterning and neuronal fate determination in central nervous system.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NKX6-1 antibody 20974-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |