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验证数据展示

  • IHC staining of mouse spleen using 31984-1-AP

    IHC staining of mouse spleen using 31984-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31984-1-AP (NKp46/NCR1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse spleen using 31984-1-AP

    IHC staining of mouse spleen using 31984-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31984-1-AP (NKp46/NCR1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NKp46/NCR1 Polyclonal antibody

NKp46/NCR1 Polyclonal Antibody for IHC, ELISA
Cat No. 31984-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

IHC, ELISA

别名

Ncr1, Natural killer cell p46-related protein, mNKp46, Lymphocyte antigen 94, Ly94

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31984-1-AP targets NKp46/NCR1 in IHC, ELISA applications and shows reactivity with mouse samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 natural cytotoxicity triggering receptor 1
别名 Ncr1, Natural killer cell p46-related protein, mNKp46, Lymphocyte antigen 94, Ly94
计算分子量37kDa
GenBank蛋白编号NM_010746.3
基因名称 NKp46/NCR1
Gene ID (NCBI) 17086
RRIDAB_3670165
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ8C567
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NKp46, also known as NCR1 or CD335, is an Ig-like superfamily cell surface receptor that is highly conserved in mammals (PMID: 22021440). NKp46 is a type I transmembrane glycoprotein consisting of two extracellular Ig-like domains, a transmembrane domain, and an intracellular tail (PMID: 9730896). It is expressed on NK cells, rare T-cell subsets and a mucosal population of NKp46+ innate lymphoid cells (PMID: 9730896; 22021440). NKp46 is the major triggering receptor involved in the natural cytotoxicity (PMID: 10359120; 10092106; 15356098).

实验方案

Product Specific Protocols
IHC protocol for NKp46/NCR1 antibody 31984-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols