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NLGN3 Polyclonal antibody

NLGN3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29319-1-AP

Print datasheet

Synonyms

NL3, Neuroligin-3, neuroligin 3, HNL3, AUTSX1

验证数据展示

  • WB analysis using 29319-1-AP

    WB analysis using 29319-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29319-1-AP (NLGN3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 29319-1-AP

    IHC staining of mouse brain using 29319-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29319-1-AP (NLGN3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 29319-1-AP

    IHC staining of mouse brain using 29319-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29319-1-AP (NLGN3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29319-1-AP targets NLGN3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen NLGN3 fusion protein Ag30536 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name neuroligin 3
Synonyms NL3, Neuroligin-3, neuroligin 3, HNL3, AUTSX1
Calculated Molecular Weight 94 kDa
Observed Molecular Weight90-110 kDa
GenBank Accession NumberBC051715
Gene Symbol NLGN3
Gene ID (NCBI) 54413
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NZ94
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Neuroligin3 (NLGN3) belongs to the neuroligin family, a class of postsynaptic cell adhesion molecules that regulate synapse organization and dendritic outgrowth, and NLGN3 missense variants have previously been associated with autistic spectrum disorder (ASD).

实验方案

Product Specific Protocols
WB protocol for NLGN3 antibody 29319-1-APDownload protocol
IHC protocol for NLGN3 antibody 29319-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols