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NLN Polyclonal antibody

NLN Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 14763-1-AP

Print datasheet

Synonyms

AGTBP, Angiotensin binding protein, KIAA1226, MEP, Microsomal endopeptidase, Mitochondrial oligopeptidase M, Neurolysin, mitochondrial, Neurotensin endopeptidase, NLN

验证数据展示

  • WB analysis of mouse ovary using 14763-1-AP

    WB analysis of mouse ovary using 14763-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 14763-1-AP (NLN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 14763-1-AP

    IHC staining of mouse testis using 14763-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14763-1-AP (NLN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse ovary tissue, HepG2 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14763-1-AP targets NLN in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen NLN fusion protein Ag6524 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name neurolysin (metallopeptidase M3 family)
Synonyms AGTBP, Angiotensin binding protein, KIAA1226, MEP, Microsomal endopeptidase, Mitochondrial oligopeptidase M, Neurolysin, mitochondrial, Neurotensin endopeptidase, NLN
Calculated Molecular Weight 81 kDa
Observed Molecular Weight 75 kDa
GenBank Accession NumberBC001644
Gene Symbol NLN
Gene ID (NCBI) 57486
RRIDAB_2151973
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BYT8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NLN(Neurolysin, mitochondrial) is also named as AGTBP, KIAA1226 and belongs to the peptidase M3 family. It is an enzyme involved in the metabolic inactivation of bioactive peptides and functions as a monomer of molecular mass 78 kDa. It also can be dected the molecular weight between 70 kDa and 85 kDa according to the reports (PMID:10095765, 11092934). The full length protein has a transit peptide with 37 amino acids.

实验方案

Product Specific Protocols
WB protocol for NLN antibody 14763-1-APDownload protocol
IHC protocol for NLN antibody 14763-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols