验证数据展示
经过测试的应用
| Positive WB detected in | Daudi cells, Jurkat cells, Raji cells, Ramos cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
30455-1-AP targets NLRC3 in WB, ELISA applications and shows reactivity with Human samples.
| Tested Applications | WB, ELISA Application Description |
| Tested Reactivity | Human |
| Immunogen | NLRC3 fusion protein Ag32323 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | NLR family, CARD domain containing 3 |
| Synonyms | CARD15 like protein, Caterpiller protein 16.2, CLR16.2, FLJ00348, NLRC3, NOD3, Protein NLRC3 |
| Calculated Molecular Weight | 115 kDa |
| Observed Molecular Weight | 100-110 kDa |
| GenBank Accession Number | NM_178844 |
| Gene Symbol | NLRC3 |
| Gene ID (NCBI) | 197358 |
| RRID | AB_3086322 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q7RTR2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NLRC3 is a member of the pattern recognition receptors nucleotide-binding oligomerization domain (NOD)-like receptors (NLRs) family, and plays a pivotal regulatory role in modulating the activation of immune cells. In macrophages, NLRC3 inhibits the activation of the NF-κB signaling pathway, the STING/TBK1 signaling pathway, and the formation of the inflammasome. In the context of T cells immune response, NLRC3 prevents the activation of T cells by regulating the function of dendritic cells and directly influencing the function of T cells.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NLRC3 antibody 30455-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |