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验证数据展示

  • WB analysis using 30998-1-AP

    WB analysis using 30998-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30998-1-AP (NLRP12 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of rat liver using 30998-1-AP

    IP experiment of rat liver using 30998-1-AP

    IP result of anti-NLRP12 (IP:30998-1-AP, 4ug; Detection:30998-1-AP 1:500) with rat liver tissue lysate 2695 ug.

NLRP12 Polyclonal antibody

NLRP12 Polyclonal Antibody for WB, IP, ELISA
Cat No. 30998-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA

别名

PYPAF7, PAN6, NALP12, NACHT, LRR and PYD domains-containing protein 12, Monarch-1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse liver tissue, rat liver tissue
Positive IP detected inrat liver tissue, PC-3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30998-1-AP targets NLRP12 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 NLRP12 fusion protein Ag34281 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NLR family, pyrin domain containing 12
别名 PYPAF7, PAN6, NALP12, NACHT, LRR and PYD domains-containing protein 12, Monarch-1
计算分子量 1061 aa, 120 kDa
观测分子量 95-110 kDa
GenBank蛋白编号BC028069
基因名称 NLRP12
Gene ID (NCBI) 91662
RRIDAB_3669811
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP59046
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NACHT, LRR and PYD domains-containing protein 12 (NLRP12) is also named as Monarch-1, NALP12, PYPAF7 and RNO. NLRP12 is as an essential cytosolic sensor for heme plus PAMPs-mediated PANoptosis, inflammation, and pathology (PMID: 37267949). NLRP12 drives inflammasome and PANoptosome activation, cell death, and inflammation in response to heme plus PAMPs or TNF. TLR2/4-mediated signaling through IRF1 induced Nlrp12 expression, which led to inflammasome formation to induce maturation of IL-1β and IL-18 (PMID: 37267949). NLRP12, a pyrin-containing NLR protein, is a negative regulator of innate immune activation and type I interferon (IFN-I) production (PMID: 36719379).

实验方案

Product Specific Protocols
WB protocol for NLRP12 antibody 30998-1-APDownload protocol
IP protocol for NLRP12 antibody 30998-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols