• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 30998-1-AP

    WB analysis using 30998-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30998-1-AP (NLRP12 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of rat liver using 30998-1-AP

    IP experiment of rat liver using 30998-1-AP

    IP result of anti-NLRP12 (IP:30998-1-AP, 4ug; Detection:30998-1-AP 1:500) with rat liver tissue lysate 2695 ug.

NLRP12 Polyclonal antibody

NLRP12 Polyclonal Antibody for WB, IP, ELISA
Cat No. 30998-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

PYPAF7, PAN6, NALP12, NACHT, LRR and PYD domains-containing protein 12, Monarch-1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inmouse liver tissue, rat liver tissue
Positive IP detected inrat liver tissue, PC-3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30998-1-AP targets NLRP12 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen NLRP12 fusion protein Ag34281 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name NLR family, pyrin domain containing 12
Synonyms PYPAF7, PAN6, NALP12, NACHT, LRR and PYD domains-containing protein 12, Monarch-1
Calculated Molecular Weight 1061 aa, 120 kDa
Observed Molecular Weight 95-110 kDa
GenBank Accession NumberBC028069
Gene Symbol NLRP12
Gene ID (NCBI) 91662
RRIDAB_3669811
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP59046
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NACHT, LRR and PYD domains-containing protein 12 (NLRP12) is also named as Monarch-1, NALP12, PYPAF7 and RNO. NLRP12 is as an essential cytosolic sensor for heme plus PAMPs-mediated PANoptosis, inflammation, and pathology (PMID: 37267949). NLRP12 drives inflammasome and PANoptosome activation, cell death, and inflammation in response to heme plus PAMPs or TNF. TLR2/4-mediated signaling through IRF1 induced Nlrp12 expression, which led to inflammasome formation to induce maturation of IL-1β and IL-18 (PMID: 37267949). NLRP12, a pyrin-containing NLR protein, is a negative regulator of innate immune activation and type I interferon (IFN-I) production (PMID: 36719379).

实验方案

Product Specific Protocols
WB protocol for NLRP12 antibody 30998-1-APDownload protocol
IP protocol for NLRP12 antibody 30998-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols