• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 83123-6-RR

    WB analysis using 83123-6-RR

    Mouse liver tissue was subjected to SDS PAGE followed by western blot with 83123-6-RR (NLRP12 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83123-6-RR

    Affinity and Kinetic Characterization of 83123-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83123-6-RR against Human NLRP12 were performed. The affinity constant is 0.236 nM.

NLRP12 Recombinant antibody

NLRP12 Recombinant Antibody for WB, ELISA
Cat No. 83123-6-RR

Print datasheet

CloneNo. 241838E11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

PAN6, NALP12, NACHT, LRR and PYD domains-containing protein 12, Monarch-1, Monarch 1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inmouse liver tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83123-6-RR targets NLRP12 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse
Immunogen NLRP12 fusion protein Ag34281 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name NLR family, pyrin domain containing 12
Synonyms PAN6, NALP12, NACHT, LRR and PYD domains-containing protein 12, Monarch-1, Monarch 1
Calculated Molecular Weight 1061 aa, 120 kDa
Observed Molecular Weight 95-110 kDa
GenBank Accession NumberBC028069
Gene Symbol NLRP12
Gene ID (NCBI) 91662
RRIDAB_3670832
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP59046
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NACHT, LRR and PYD domains-containing protein 12 (NLRP12) is also named as Monarch-1, NALP12, PYPAF7 and RNO. NLRP12 is as an essential cytosolic sensor for heme plus PAMPs-mediated PANoptosis, inflammation, and pathology (PMID: 37267949). NLRP12 drives inflammasome and PANoptosome activation, cell death, and inflammation in response to heme plus PAMPs or TNF. TLR2/4-mediated signaling through IRF1 induced Nlrp12 expression, which led to inflammasome formation to induce maturation of IL-1β and IL-18 (PMID: 37267949). NLRP12, a pyrin-containing NLR protein, is a negative regulator of innate immune activation and type I interferon (IFN-I) production (PMID: 36719379).

实验方案

Product Specific Protocols
WB protocol for NLRP12 antibody 83123-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols