验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, human lung tissue |
| Positive IP detected in | A549 cells |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15182-1-AP targets NLRP2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | NLRP2 fusion protein Ag7167 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | NLR family, pyrin domain containing 2 |
| 别名 | CLR19.9, NALP2, NBS1, NLRP2, PAN1, PYPAF2 |
| 计算分子量 | 121 kDa |
| 观测分子量 | 105-110 kDa |
| GenBank蛋白编号 | BC001039 |
| 基因名称 | NLRP2 |
| Gene ID (NCBI) | 55655 |
| RRID | AB_2298531 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NX02 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NLRP2 antibody 15182-1-AP | Download protocol |
| IHC protocol for NLRP2 antibody 15182-1-AP | Download protocol |
| IP protocol for NLRP2 antibody 15182-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Immunol Macrophage IL-1β contributes to tumorigenesis through paracrine AIM2 inflammasome activation in the tumor microenvironment | ||
Brain Behav Immun Kynurenine regulates NLRP2 inflammasome in astrocytes and its implications in depression.
| ||
Eur J Immunol NLRP2 negatively regulates antiviral immunity by interacting with TBK1.
| ||
Front Microbiol Porphyromonas gingivalis-Induced NLRP3 Inflammasome Activation and Its Downstream Interleukin-1β Release Depend on Caspase-4. | ||
Mol Cell Endocrinol Glucagon-like peptide-1 analogs mitigate neuroinflammation in Alzheimer's disease by suppressing NLRP2 activation in astrocytes | ||
J Neuroimmunol A selective NLRP3 inflammasome inhibitor attenuates behavioral deficits and neuroinflammation in a mouse model of Parkinson's disease. |
