NLRP3 Polyclonal antibody
NLRP3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, IF/ICC, IP, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | Jurkat cells, THP-1 cells |
Positive IP detected in | THP-1 cells |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
27458-1-AP targets NLRP3 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, IHC, IF, CoIP |
Tested Reactivity | human |
Cited Reactivity | human, pig |
Immunogen | NLRP3 fusion protein Ag26272 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | NLR family, pyrin domain containing 3 |
Synonyms | NACHT, LRR and PYD domains-containing protein 3, EC:3.6.4.-, Cold-induced autoinflammatory syndrome 1 protein, CIAS1, C1orf7 |
Calculated Molecular Weight | 118 kDa |
Observed Molecular Weight | 110 kDa |
GenBank Accession Number | NM_001079821 |
Gene Symbol | NLRP3 |
Gene ID (NCBI) | 114548 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q96P20 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for NLRP3 antibody 27458-1-AP | Download protocol |
IHC protocol for NLRP3 antibody 27458-1-AP | Download protocol |
IF protocol for NLRP3 antibody 27458-1-AP | Download protocol |
IP protocol for NLRP3 antibody 27458-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Adv Sci (Weinh) Inflammatory Fibroblast-Like Synoviocyte-Derived Exosomes Aggravate Osteoarthritis via Enhancing Macrophage Glycolysis | ||
J Physiol Pharmacol Prodigiosin improves acute lung injury in a rat model of rheumatoid arthritis via down-regulating the nuclear factor kappaB/nucleotide-binding domain, leucine-rich-containing family, pyrin domain-containing-3 signaling pathway | ||
Phytomedicine Achyranthoside D attenuates chondrocyte loss and inflammation in osteoarthritis via targeted regulation of Wnt3a | ||
Biomed Pharmacother Protective role of hydrogen sulfide against diabetic cardiomyopathy by inhibiting pyroptosis and myocardial fibrosis | ||
Antioxidants (Basel) Epimedium Aqueous Extract Ameliorates Cerebral Ischemia/Reperfusion Injury through Inhibiting ROS/NLRP3-Mediated Pyroptosis | ||
Ecotoxicol Environ Saf Triclosan induces liver injury in long-life exposed mice via activation of TLR4/NF-κB/NLRP3 pathway |