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NLRP3 Monoclonal antibody, PBS Only (Capture)

NLRP3 Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3H1A7

Cat no : 68102-1-PBS

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Synonyms

EC:3.6.4.-, Cold-induced autoinflammatory syndrome 1 protein, CIAS1, C1orf7, Angiotensin/vasopressin receptor AII/AVP-like



Planning an IHC experiment? We recommend our IHCeasy NLRP3 Ready-To-Use IHC Kit. NLRP3 primary antibody included.

产品信息

68102-1-PBS targets NLRP3 as part of a matched antibody pair:

MP50203-1: 68102-1-PBS capture and 68102-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen NLRP3 fusion protein Ag26289 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name NLR family, pyrin domain containing 3
Synonyms EC:3.6.4.-, Cold-induced autoinflammatory syndrome 1 protein, CIAS1, C1orf7, Angiotensin/vasopressin receptor AII/AVP-like
Calculated Molecular Weight 118 kDa
Observed Molecular Weight110 kDa
GenBank Accession NumberNM_001079821
Gene Symbol NLRP3
Gene ID (NCBI) 114548
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NALP3, also named as C1orf7, CIAS1 and PYPAF1, belongs to the NLRP family. NLRP3, a key and eponymous component of the NLRP3 inflammasome, plays a crucial role in innate immunity and inflammation. NALP3 may function as an inducer of apoptosis. It interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling.NALP3 inhibits TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. NALP3 activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18. Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1) which also known as familial cold urticaria. Defects in NLRP3 are a cause of Muckle-Wells syndrome (MWS) which is urticaria-deafness-amyloidosis syndrome. Defects in NLRP3 are the cause of chronic infantile neurologic cutaneous and articular syndrome (CINCA) which also known as neonatal onset multisystem inflammatory disease (NOMID).