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NLRP7 Polyclonal antibody

NLRP7 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27897-1-AP

Print datasheet

Synonyms

CLR19.4, HYDM, NALP7, NLRP7, NOD12, PAN7, PYPAF3

验证数据展示

  • WB analysis using 27897-1-AP

    WB analysis using 27897-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27897-1-AP (NLRP7 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human spleen using 27897-1-AP

    IHC staining of human spleen using 27897-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 27897-1-AP (NLRP7 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human spleen using 27897-1-AP

    IHC staining of human spleen using 27897-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 27897-1-AP (NLRP7 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inK-562 cells, THP-1 cells
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27897-1-AP targets NLRP7 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human
Immunogen NLRP7 fusion protein Ag27465 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name NLR family, pyrin domain containing 7
Synonyms CLR19.4, HYDM, NALP7, NLRP7, NOD12, PAN7, PYPAF3
Calculated Molecular Weight112 kDa, 115 kDa, 118 kDa
Observed Molecular Weight112 kDa
GenBank Accession NumberBC109125
Gene Symbol NLRP7
Gene ID (NCBI) 199713
RRIDAB_3086005
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WX94
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NLRP7, also called NALP7 or PYPAF3, is a member of the NOD-like receptors (NLR), a family of proteins that plays a crucial role in the innate immune response. NLRP7 has been reported to emerge from NLRP2 gene. Similar to all inflammasomes, NLRP7 contributes to both pro- and an anti-inflammatory processes, depending on whether it functions in an inflammasome-dependent or independent pathway. The function of NLRP7 inflammasome has also been reported to depend on the master regulatory transcription factor protein that controls cellular inflammation, the NF-kB. In addition to its pro- and anti-inflammatory actions, NLRP7 plays a role in restricting intracellular bacterial replication and can also induce inflammasome assembly upon specific stimulation by FSL-1 (diacylated lipoprotein).

实验方案

Product Specific Protocols
WB protocol for NLRP7 antibody 27897-1-APDownload protocol
IHC protocol for NLRP7 antibody 27897-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols