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验证数据展示

  • WB analysis using 27897-1-AP

    WB analysis using 27897-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27897-1-AP (NLRP7 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human spleen using 27897-1-AP

    IHC staining of human spleen using 27897-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 27897-1-AP (NLRP7 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human spleen using 27897-1-AP

    IHC staining of human spleen using 27897-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 27897-1-AP (NLRP7 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NLRP7 Polyclonal antibody

NLRP7 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27897-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, IHC, ELISA

别名

CLR19.4, HYDM, NALP7, NLRP7, NOD12, PAN7, PYPAF3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, THP-1 cells
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27897-1-AP targets NLRP7 in WB, IHC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human
免疫原 NLRP7 fusion protein Ag27465 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NLR family, pyrin domain containing 7
别名 CLR19.4, HYDM, NALP7, NLRP7, NOD12, PAN7, PYPAF3
计算分子量112 kDa, 115 kDa, 118 kDa
观测分子量112 kDa
GenBank蛋白编号BC109125
基因名称 NLRP7
Gene ID (NCBI) 199713
RRIDAB_3086005
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WX94
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NLRP7, also called NALP7 or PYPAF3, is a member of the NOD-like receptors (NLR), a family of proteins that plays a crucial role in the innate immune response. NLRP7 has been reported to emerge from NLRP2 gene. Similar to all inflammasomes, NLRP7 contributes to both pro- and an anti-inflammatory processes, depending on whether it functions in an inflammasome-dependent or independent pathway. The function of NLRP7 inflammasome has also been reported to depend on the master regulatory transcription factor protein that controls cellular inflammation, the NF-kB. In addition to its pro- and anti-inflammatory actions, NLRP7 plays a role in restricting intracellular bacterial replication and can also induce inflammasome assembly upon specific stimulation by FSL-1 (diacylated lipoprotein).

实验方案

Product Specific Protocols
WB protocol for NLRP7 antibody 27897-1-APDownload protocol
IHC protocol for NLRP7 antibody 27897-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols