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NMD3 Polyclonal antibody

NMD3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 16060-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

NMD 3, hNMD3, CGI-07, CGI07, CGI 07

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHepG2 cells, L02 cells, mouse liver tissue, rat liver tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman breast cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16060-1-AP targets NMD3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 NMD3 fusion protein Ag8894 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NMD3 homolog (S. cerevisiae)
别名 NMD 3, hNMD3, CGI-07, CGI07, CGI 07
计算分子量 503 aa, 58 kDa
观测分子量 58 kDa
GenBank蛋白编号BC013317
基因名称 NMD3
Gene ID (NCBI) 51068
RRIDAB_2282830
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96D46
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human NMD3 gene encodes 60S ribosomal export protein which was found in a 60S ribosomal subunit export complex with RAN and XPO1. This nucleocytoplasmic shuttling protein NMD3 is an adaptor for export of the 60S ribosomal subunit from the nucleus. NMD3 contains a CRM-1-dependent leucine-rich nuclear export signal (NES) and a dispersed nuclear localization signal (NLS), the basic region of which is also required for nucleolar accumulation. NMD3 are required for nuclear export of the 60S ribosomal subunit in yeast and vertebrate cells, recent finding has also revealed its role in Arabidopsis thaliana.

实验方案

Product Specific Protocols
WB protocol for NMD3 antibody 16060-1-APDownload protocol
IHC protocol for NMD3 antibody 16060-1-APDownload protocol
IF protocol for NMD3 antibody 16060-1-APDownload protocol
IP protocol for NMD3 antibody 16060-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Stem Cell

HectD1 controls hematopoietic stem cell regeneration by coordinating ribosome assembly and protein synthesis.

Authors - Kaosheng Lv
mouseWB

Genes Dev

Uncoupling of GTP hydrolysis from eIF6 release on the ribosome causes Shwachman-Diamond syndrome.

Authors - Andrew J Finch
humanWB

Blood

Defective ribosome assembly in Shwachman-Diamond syndrome.

Authors - Wong Chi C CC
humanIF

Biomolecules

Mutations of Key Functional Residues in CRM1/XPO1 Differently Alter Its Intranuclear Localization and the Nuclear Export of Endogenous Cargos

Authors - Miren Josu Omaetxebarria
humanIF

Nucleic Acids Res

The induction of p53 correlates with defects in the production, but not the levels, of the small ribosomal subunit and stalled large ribosomal subunit biogenesis

Authors - Matthew John Eastham
humanWB

Cancer Lett

RNA-binding protein hnRNPU regulates multiple myeloma resistance to selinexor

Authors - Xin Wang
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