NME1 Polyclonal antibody

NME1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 11086-2-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, ELISA and More (3)

NB, Granzyme A-activated DNase, Granzyme A activated DNase, GAAD, EC:2.7.4.6

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, multi-cells
Positive IP detected inA549 cells
Positive IHC detected inhuman lung cancer tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy NME1 Ready-To-Use IHC Kit. NME1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11086-2-AP targets NME1 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, CoIP, ChIP, IF
经测试反应性 human
文献引用反应性human
免疫原 NME1 fusion protein Ag1548 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 non-metastatic cells 1, protein (NM23A) expressed in
别名 NB, Granzyme A-activated DNase, Granzyme A activated DNase, GAAD, EC:2.7.4.6
计算分子量 17 kDa
观测分子量 16-20 kDa
GenBank蛋白编号BC018994
基因名称 NME1
Gene ID (NCBI) 4830
RRIDAB_2152689
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP15531
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NME1 is also named as NDPKA(Nucleoside diphosphate kinase A), NM23(Metastasis inhibition factor nm23),GAAD(Granzyme A-activated DNase) and belongs to the NDK family.It is a major role in the synthesis of nucleoside triphosphates other than ATP and required for neural development including neural patterning and cell fate determination.It has 3 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for NME1 antibody 11086-2-APDownload protocol
IHC protocol for NME1 antibody 11086-2-APDownload protocol
IF protocol for NME1 antibody 11086-2-APDownload protocol
IP protocol for NME1 antibody 11086-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

Regulation of local GTP availability controls RAC1 activity and cell invasion

Authors - Anna Bianchi-Smiraglia
humanWB

Redox Biol

Regulation of metastasis suppressor NME1 by a key metabolic cofactor coenzyme A.

Authors - Bess Yi Kun Yu
humanCoIP

Int J Mol Sci

APEX2-Mediated Proximity Labeling Resolves the DDIT4-Interacting Proteome.

Authors - Marianna Naki
humanIF

J Transl Med

Identification and validation of smoking-related genes in lung adenocarcinoma using an in vitro carcinogenesis model and bioinformatics analysis.

Authors - Jin Wang
humanWB

J Proteomics

Proteomic profiling of human decidual immune proteins during Toxoplasma gondii infection.

Authors - Dan Zhang
humanIF

Oncol Rep

LINC01816 promotes the migration, invasion and epithelial‑mesenchymal transition of thyroid carcinoma cells by sponging miR‑34c‑5p and regulating CRABP2 expression levels.

Authors - Hongyuan Zhao
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