NME1 Polyclonal antibody

NME1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA and More (3)

Conjugate

Unconjugated

Publications

Cat no : 11086-2-AP

Print datasheet

Synonyms

NB, Granzyme A-activated DNase, Granzyme A activated DNase, GAAD, EC:2.7.4.6

验证数据展示

WB analysis of HeLa using 11086-2-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11086-2-AP (NME1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IHC staining of human lung cancer using 11086-2-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 11086-2-AP (NME1 antibody) at dilution of 1:100 (under 10x lens).
Ready-to-use IHC kits available with NME1 antibody included
IHC staining of human lung cancer using 11086-2-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 11086-2-AP (NME1 antibody) at dilution of 1:100 (under 40x lens).
Ready-to-use IHC kits available with NME1 antibody included
IHC staining of human liver using 11086-2-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11086-2-AP (NME1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with NME1 antibody included
IHC staining of human liver using 11086-2-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11086-2-AP (NME1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with NME1 antibody included
WB analysis of multi-cells using 11086-2-AP
WB result of 10086-2-AP (NME1 antibody).
IP experiment of A549 using 11086-2-AP
IP result of anti-NME1 (IP:11086-2-AP, 4ug; Detection:11086-2-AP 1:500) with A549 cells lysate 2800ug.
IF Staining of MCF-7 using 11086-2-AP
Immunofluorescent analysis of MCF-7 cells using 11086-2-AP (NME1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 11086-2-AP

Size

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经过测试的应用

Positive WB detected in	HeLa cells, multi-cells
Positive IP detected in	A549 cells
Positive IHC detected in	human lung cancer tissue, human pancreas cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells

Planning an IHC experiment? We recommend our IHCeasy NME1 Ready-To-Use IHC Kit. NME1 primary antibody included.

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WB	See 8 publications below
IHC	See 2 publications below
IF	See 3 publications below
CoIP	See 1 publications below
ChIP	See 1 publications below

产品信息

11086-2-AP targets NME1 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human samples.

Tested Applications	WB, IHC, IF/ICC, IP, ELISA Application Description
Cited Applications	WB, IHC, CoIP, ChIP, IF
Tested Reactivity	human
Cited Reactivity	human
Immunogen	NME1 fusion protein Ag1548 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	non-metastatic cells 1, protein (NM23A) expressed in
Synonyms	NB, Granzyme A-activated DNase, Granzyme A activated DNase, GAAD, EC:2.7.4.6
Calculated Molecular Weight	17 kDa
Observed Molecular Weight	16-20 kDa
GenBank Accession Number	BC018994
Gene Symbol	NME1
Gene ID (NCBI)	4830
RRID	AB_2152689
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P15531
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

NME1 is also named as NDPKA(Nucleoside diphosphate kinase A), NM23(Metastasis inhibition factor nm23),GAAD(Granzyme A-activated DNase) and belongs to the NDK family.It is a major role in the synthesis of nucleoside triphosphates other than ATP and required for neural development including neural patterning and cell fate determination.It has 3 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for NME1 antibody 11086-2-AP	Download protocol
IHC protocol for NME1 antibody 11086-2-AP	Download protocol
IF protocol for NME1 antibody 11086-2-AP	Download protocol
IP protocol for NME1 antibody 11086-2-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB	Nat Commun Regulation of local GTP availability controls RAC1 activity and cell invasion Authors - Anna Bianchi-Smiraglia View Article
human	WB	Redox Biol Regulation of metastasis suppressor NME1 by a key metabolic cofactor coenzyme A. Authors - Bess Yi Kun Yu View Article
human	CoIP	Int J Mol Sci APEX2-Mediated Proximity Labeling Resolves the DDIT4-Interacting Proteome. Authors - Marianna Naki View Article
human	IF	J Transl Med Identification and validation of smoking-related genes in lung adenocarcinoma using an in vitro carcinogenesis model and bioinformatics analysis. Authors - Jin Wang View Article
human	WB	J Proteomics Proteomic profiling of human decidual immune proteins during Toxoplasma gondii infection. Authors - Dan Zhang View Article
human	IF	Oncol Rep LINC01816 promotes the migration, invasion and epithelial‑mesenchymal transition of thyroid carcinoma cells by sponging miR‑34c‑5p and regulating CRABP2 expression levels. Authors - Hongyuan Zhao View Article

NME1 Polyclonal antibody

NME1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

验证数据展示

WB analysis of HeLa using 11086-2-AP

IHC staining of human lung cancer using 11086-2-AP

IHC staining of human lung cancer using 11086-2-AP

IHC staining of human liver using 11086-2-AP

IHC staining of human liver using 11086-2-AP

WB analysis of multi-cells using 11086-2-AP

IP experiment of A549 using 11086-2-AP

IF Staining of MCF-7 using 11086-2-AP

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Regulation of local GTP availability controls RAC1 activity and cell invasion

Regulation of metastasis suppressor NME1 by a key metabolic cofactor coenzyme A.

APEX2-Mediated Proximity Labeling Resolves the DDIT4-Interacting Proteome.

Identification and validation of smoking-related genes in lung adenocarcinoma using an in vitro carcinogenesis model and bioinformatics analysis.

Proteomic profiling of human decidual immune proteins during Toxoplasma gondii infection.

LINC01816 promotes the migration, invasion and epithelial‑mesenchymal transition of thyroid carcinoma cells by sponging miR‑34c‑5p and regulating CRABP2 expression levels.