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NME2 Polyclonal antibody, PBS Only

NME2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 20493-1-PBS

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Synonyms

验证数据展示

  • WB analysis of mouse kidney using 20493-1-AP (same clone as 20493-1-PBS)

    WB analysis of mouse kidney using 20493-1-AP (same clone as 20493-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20493-1-AP (NME2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20493-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 20493-1-AP (same clone as 20493-1-PBS)

    IHC staining of mouse kidney using 20493-1-AP (same clone as 20493-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20493-1-AP (NME2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20493-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 20493-1-AP (same clone as 20493-1-PBS)

    IF Staining of HEK-293 using 20493-1-AP (same clone as 20493-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using 20493-1-AP (NME2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20493-1-PBS in a different storage buffer formulation.

产品信息

20493-1-PBS targets NME2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen NME2 fusion protein Ag13913 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name non-metastatic cells 2, protein (NM23B) expressed in
Synonyms
Calculated Molecular Weight 152 aa, 17 kDa
Observed Molecular Weight 17 kDa
GenBank Accession NumberBC002476
Gene Symbol NME2
Gene ID (NCBI) 4831
RRIDAB_10695634
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP22392
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NME2(non-metastatic cells 2, protein) is also named as NDKB(Nucleoside diphosphate kinase B), NM23B and belongs to the NDK family. It is involved in the phosphorylation of nucleoside diphosphates and interacts with membrane receptor and constituting a novel molecular regulatory mechanism of GPCR endocytosis. This protein has 2 isoforms produced by alternative splicing.