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验证数据展示

  • WB analysis using 15136-1-AP

    WB analysis using 15136-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15136-1-AP (NME3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 15136-1-AP

    IP experiment of HEK-293 using 15136-1-AP

    IP result of anti-NME3 (IP:15136-1-AP, 4ug; Detection:15136-1-AP 1:300) with HEK-293 cells lysate 2400 ug.

  • IF Staining of HeLa using 15136-1-AP

    IF Staining of HeLa using 15136-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NME3 antibody (15136-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

  • IHC staining of human breast cancer using 15136-1-AP

    IHC staining of human breast cancer using 15136-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15136-1-AP (NME3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 15136-1-AP

    IHC staining of human breast cancer using 15136-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15136-1-AP (NME3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

NME3 Polyclonal antibody

NME3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 15136-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

别名

NDP kinase 3, NDK 3, KIAA0516, EC:2.7.4.6, DR-nm23

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, MCF-7 cells, mouse kidney tissue, K-562 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, MCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15136-1-AP targets NME3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性human, zebrafish
免疫原 NME3 fusion protein Ag7284 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 non-metastatic cells 3, protein expressed in
别名 NDP kinase 3, NDK 3, KIAA0516, EC:2.7.4.6, DR-nm23
计算分子量 19 kDa
观测分子量 18 kDa
GenBank蛋白编号BC000250
基因名称 NME3
Gene ID (NCBI) 4832
RRIDAB_10858322
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13232
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NME3 (NME/NM23 Nucleoside Diphosphate Kinase 3), also named as NM23-H3; DR-NM23, is a member of the nucleoside diphosphate kinase (NDPK) family that binds to the mitochondrial outer membrane to stimulate mitochondrial fusion. The depletion of NME3 will cause dysfunction in mitochondrial dynamics. NME3 has been found to facilitate DNA-repair mechanisms binds to several NPHPs, including NEK8, CEP164, and ankyrin repeat and sterile α motif domain-containing 6 (ANKS6).

实验方案

Product Specific Protocols
WB protocol for NME3 antibody 15136-1-APDownload protocol
IHC protocol for NME3 antibody 15136-1-APDownload protocol
IF protocol for NME3 antibody 15136-1-APDownload protocol
IP protocol for NME3 antibody 15136-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,zebrafishIF,WB

J Biol Chem

The nucleoside diphosphate kinase NME3 associates with nephronophthisis proteins, and is required for ciliary function during renal development.

Authors - Sylvia Hoff
  • KD Validated
View Article