验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue, human kidney tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12923-1-AP targets NME5 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | NME5 fusion protein Ag3610 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | non-metastatic cells 5, protein expressed in (nucleoside-diphosphate kinase) |
| 别名 | IPIA beta, NDK H 5, NDP kinase homolog 5, NME5, Testis specific nm23 homolog |
| 计算分子量 | 212 aa, 24 kDa |
| 观测分子量 | 24 kDa |
| GenBank蛋白编号 | BC026182 |
| 基因名称 | NME5 |
| Gene ID (NCBI) | 8382 |
| RRID | AB_2152973 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P56597 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NME5(Nucleoside diphosphate kinase homolog 5) belongs to the NDK family. Its expression plays a critical role in spermiogenesis by increasing the cellular levels of GPX5 to eliminate reactive oxygen species. NME5 may have specific functions in the phosphotransfer network involved in spermiogenesis and flagellar movement.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NME5 antibody 12923-1-AP | Download protocol |
| IHC protocol for NME5 antibody 12923-1-AP | Download protocol |
| IP protocol for NME5 antibody 12923-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Curr Biol CCDC176 stabilizes microtubule doublets 1 and 9 to ensure proper sperm movement | ||
J Hazard Mater TCDD promotes liver fibrosis through disordering systemic and hepatic iron homeostasis. | ||
Int J Cancer Additive effect of the AZGP1, PIP, S100A8, and UBE2C molecular biomarkers improves outcome prediction in breast carcinoma. | ||
Cell Rep Differential requirements of IQUB for the assembly of radial spoke 1 and the motility of mouse cilia and flagella | ||
Cell Mol Life Sci CCDC146 is required for sperm flagellum biogenesis and male fertility in mice | ||
Sci China Life Sci Adenylate kinase phosphate energy shuttle underlies energetic communication in flagellar axonemes |
