• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

NME6 Polyclonal antibody

NME6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10250-1-AP

Print datasheet

Synonyms

IPIA ALPHA, NDK 6, NDP kinase 6, NME6

验证数据展示

  • IHC staining of human colon cancer using 10250-1-AP

    IHC staining of human colon cancer using 10250-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10250-1-AP (NME6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 10250-1-AP

    IHC staining of human colon cancer using 10250-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10250-1-AP (NME6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of LNCaP using 10250-1-AP

    WB analysis of LNCaP using 10250-1-AP

    LNCaP cells were subjected to SDS PAGE followed by western blot with 10250-1-AP (NME6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inLNCaP cells, U2OS cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10250-1-AP targets NME6 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen NME6 fusion protein Ag0315 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name non-metastatic cells 6, protein expressed in (nucleoside-diphosphate kinase)
Synonyms IPIA ALPHA, NDK 6, NDP kinase 6, NME6
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC001808
Gene Symbol NME6
Gene ID (NCBI) 10201
RRIDAB_2298600
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75414
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NME6 antibody 10250-1-APDownload protocol
IHC protocol for NME6 antibody 10250-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols