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验证数据展示

  • IHC staining of human colon cancer using 10250-1-AP

    IHC staining of human colon cancer using 10250-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10250-1-AP (NME6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 10250-1-AP

    IHC staining of human colon cancer using 10250-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10250-1-AP (NME6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of LNCaP using 10250-1-AP

    WB analysis of LNCaP using 10250-1-AP

    LNCaP cells were subjected to SDS PAGE followed by western blot with 10250-1-AP (NME6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

NME6 Polyclonal antibody

NME6 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 10250-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

IPIA ALPHA, NDK 6, NDP kinase 6, NME6

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, U2OS cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10250-1-AP targets NME6 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 NME6 fusion protein Ag0315 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 non-metastatic cells 6, protein expressed in (nucleoside-diphosphate kinase)
别名 IPIA ALPHA, NDK 6, NDP kinase 6, NME6
计算分子量 22 kDa
观测分子量 21 kDa
GenBank蛋白编号BC001808
基因名称 NME6
Gene ID (NCBI) 10201
RRIDAB_2298600
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75414
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NME6 antibody 10250-1-APDownload protocol
IHC protocol for NME6 antibody 10250-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols