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NME7 Polyclonal antibody

NME7 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26449-1-AP

Print datasheet

Synonyms

NDK 7, NDP kinase 7, NME7

验证数据展示

  • IHC staining of human brain using 26449-1-AP

    IHC staining of human brain using 26449-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26449-1-AP (NME7 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human brain using 26449-1-AP

    IHC staining of human brain using 26449-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26449-1-AP (NME7 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of mouse embryo using 26449-1-AP

    WB analysis of mouse embryo using 26449-1-AP

    mouse embryo tissue were subjected to SDS PAGE followed by western blot with 26449-1-AP (NME7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse embryo tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26449-1-AP targets NME7 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen NME7 fusion protein Ag24918 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name non-metastatic cells 7, protein expressed in (nucleoside-diphosphate kinase)
Synonyms NDK 7, NDP kinase 7, NME7
Calculated Molecular Weight 376 aa, 42 kDa
Observed Molecular Weight 38-42 kDa
GenBank Accession NumberBC006983
Gene Symbol NME7
Gene ID (NCBI) 29922
RRIDAB_2880517
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y5B8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NME7, also named as NDK7, nm23-H7, plays the major role in the synthesis of nucleoside triphosphates other than ATP. NME7 has two isoforms with MW 38-42 kDa.

实验方案

Product Specific Protocols
WB protocol for NME7 antibody 26449-1-APDownload protocol
IHC protocol for NME7 antibody 26449-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols