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验证数据展示

  • WB analysis using 28493-1-AP

    WB analysis using 28493-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28493-1-AP (NMNAT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

NMNAT1 Polyclonal antibody

NMNAT1 Polyclonal Antibody for WB, ELISA
Cat No. 28493-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (1)

Applications

WB, ELISA

Synonyms

NaMN adenylyltransferase 1, NMNAT1, PNAT 1, PNAT1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse skeletal muscle tissue
Planning an IHC experiment? We recommend our IHCeasy NMNAT1 Ready-To-Use IHC Kit. NMNAT1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

28493-1-AP targets NMNAT1 in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human, mouse
Cited Reactivityhuman, mouse
Immunogen NMNAT1 fusion protein Ag29584 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nicotinamide nucleotide adenylyltransferase 1
Synonyms NaMN adenylyltransferase 1, NMNAT1, PNAT 1, PNAT1
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC014943
Gene Symbol NMNAT1
Gene ID (NCBI) 64802
RRIDAB_3086056
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HAN9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NMNAT1 is a member of the nicotinamide-nucleotide adenylyltransferases (NMNATs) which catalyze nicotinamide adenine dinucleotide (NAD) synthesis (PMID: 28445802). NMNAT is a central enzyme in NAD biosynthesis, catalyzing the formation of NAD+ from nicotinamide mononucleotide (NMN) and ATP (PMID: 17402747). NMNAT1 is widely expressed with the highest levels in skeletal muscle, heart, and kidney(PMID: 11027696). Mutations in NMNAT1 have been shown associated with the LCA9 form of the retinal degeneration pathology Leber's congenital amaurosis (PMID: 22842229, 22842230).

实验方案

Product Specific Protocols
WB protocol for NMNAT1 antibody 28493-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB

Nat Cardiovasc Res

Mitochondrial NAD+ deficiency in vascular smooth muscle impairs collagen III turnover to trigger thoracic and abdominal aortic aneurysm

Authors - Jingjing Zhang
View Article