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验证数据展示

  • WB analysis using 15765-1-AP

    WB analysis using 15765-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15765-1-AP (NMRAL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 15765-1-AP

    WB analysis using 15765-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15765-1-AP (NMRAL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 15765-1-AP

    IHC staining of human liver using 15765-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15765-1-AP (NMRAL1 antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with NMRAL1 antibody included
  • IHC staining of human liver using 15765-1-AP

    IHC staining of human liver using 15765-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15765-1-AP (NMRAL1 antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with NMRAL1 antibody included
  • IP experiment of Jurkat using 15765-1-AP

    IP experiment of Jurkat using 15765-1-AP

    IP result of anti-NMRAL1 (IP:15765-1-AP, 4ug; Detection:15765-1-AP 1:1500) with Jurkat cells lysate 1560 ug.

NMRAL1 Polyclonal antibody

NMRAL1 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 15765-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IHC, IP, ELISA and More (1)

别名

HSCARG, NmrA-like family domain-containing protein 1, SDR48A1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, LNCaP cells, MCF-7 cells, mouse brain tissue, mouse heart tissue, mouse spleen tissue
Positive IP detected inJurkat cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy NMRAL1 Ready-To-Use IHC Kit. NMRAL1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15765-1-AP targets NMRAL1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse
文献引用反应性mouse, rat
免疫原 NMRAL1 fusion protein Ag8401 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NmrA-like family domain containing 1
别名 HSCARG, NmrA-like family domain-containing protein 1, SDR48A1
计算分子量 299 aa, 33 kDa
观测分子量33 kDa
GenBank蛋白编号BC007364
基因名称 NMRAL1
Gene ID (NCBI) 57407
RRIDAB_2153154
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HBL8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NMRAL1 antibody 15765-1-APDownload protocol
IHC protocol for NMRAL1 antibody 15765-1-APDownload protocol
IP protocol for NMRAL1 antibody 15765-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Metab

Stamp2 controls macrophage inflammation through nicotinamide adenine dinucleotide phosphate homeostasis and protects against atherosclerosis.

Authors - Henrik ten Freyhaus
View Article
ratIF

Diabetes Metab Syndr Obes

STAMP2 Attenuates Cardiac Dysfunction and Insulin Resistance in Diabetic Cardiomyopathy via NMRAL1-Mediated NF-κB Inhibition in Type 2 Diabetic Rats

Authors - Zhan Gao
View Article