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验证数据展示

  • WB analysis using 67984-1-Ig (same clone as 67984-1-PBS)

    WB analysis using 67984-1-Ig (same clone as 67984-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67984-1-Ig (NMT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67984-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67984-1-Ig (same clone as 67984-1-PBS)

    IHC staining of human breast cancer using 67984-1-Ig (same clone as 67984-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67984-1-Ig (NMT1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67984-1-PBS in a different storage buffer formulation.

  • WB analysis of SKOV-3 using 67984-1-Ig (same clone as 67984-1-PBS)

    WB analysis of SKOV-3 using 67984-1-Ig (same clone as 67984-1-PBS)

    WB result of NMT1 antibody (67984-1-Ig; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NMT1 transfected SKOV-3 cells. This data was developed using the same antibody clone with 67984-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

NMT1 Monoclonal antibody, PBS Only

NMT1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67984-1-PBS

产品说明书

CloneNo. 2D9B9

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

N myristoyltransferase 1, NMT1, Type I N myristoyltransferase

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67984-1-PBS targets NMT1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 NMT1 fusion protein Ag20541 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 N-myristoyltransferase 1
别名 N myristoyltransferase 1, NMT1, Type I N myristoyltransferase
计算分子量 496 aa, 57 kDa
观测分子量50-60 kDa
GenBank蛋白编号BC006569
基因名称 NMT1
Gene ID (NCBI) 4836
RRIDAB_2918733
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP30419
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NMT1 is a N-myristoyltransferase responsible for the transfer of myristate from CoA to an amino-terminal glycine of many eukaryotic proteins, which facilitates the targeting of proteins to membrane surfaces and is essential for viability of the organism. Insertional mutagenesis of the Nmt1 gene in Saccharomyces cerevisiae causes recessive lethality. Humans and mice possess two distinct but structurally similar enzymes, NMT1 and NMT2, ubiquitously expressed in most human and mouse tissues. Western analysis revealed that there are 4 isoforms of NMT1 with apparent molecular masses ranging from 49 to 68 kDa. In cell fractionation studies, the 68-kDa NMT1 isoform and NMT2 were present in both membrane and cytoplasmic fractions, while the smaller NMT1 isoforms were predominantly cytoplasmic.