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  • Featured Product
  • KD/KO Validated

NMT1 Monoclonal antibody, PBS Only

NMT1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D9B9

Cat no : 67984-1-PBS

Print datasheet

Synonyms

N myristoyltransferase 1, NMT1, Type I N myristoyltransferase

验证数据展示

  • WB analysis using 67984-1-Ig (same clone as 67984-1-PBS)

    WB analysis using 67984-1-Ig (same clone as 67984-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67984-1-Ig (NMT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67984-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67984-1-Ig (same clone as 67984-1-PBS)

    IHC staining of human breast cancer using 67984-1-Ig (same clone as 67984-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67984-1-Ig (NMT1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67984-1-PBS in a different storage buffer formulation.

  • WB analysis of SKOV-3 using 67984-1-Ig (same clone as 67984-1-PBS)

    WB analysis of SKOV-3 using 67984-1-Ig (same clone as 67984-1-PBS)

    WB result of NMT1 antibody (67984-1-Ig; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NMT1 transfected SKOV-3 cells. This data was developed using the same antibody clone with 67984-1-PBS in a different storage buffer formulation.

产品信息

67984-1-PBS targets NMT1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen NMT1 fusion protein Ag20541 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name N-myristoyltransferase 1
Synonyms N myristoyltransferase 1, NMT1, Type I N myristoyltransferase
Calculated Molecular Weight 496 aa, 57 kDa
Observed Molecular Weight50-60 kDa
GenBank Accession NumberBC006569
Gene Symbol NMT1
Gene ID (NCBI) 4836
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP30419
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NMT1 is a N-myristoyltransferase responsible for the transfer of myristate from CoA to an amino-terminal glycine of many eukaryotic proteins, which facilitates the targeting of proteins to membrane surfaces and is essential for viability of the organism. Insertional mutagenesis of the Nmt1 gene in Saccharomyces cerevisiae causes recessive lethality. Humans and mice possess two distinct but structurally similar enzymes, NMT1 and NMT2, ubiquitously expressed in most human and mouse tissues. Western analysis revealed that there are 4 isoforms of NMT1 with apparent molecular masses ranging from 49 to 68 kDa. In cell fractionation studies, the 68-kDa NMT1 isoform and NMT2 were present in both membrane and cytoplasmic fractions, while the smaller NMT1 isoforms were predominantly cytoplasmic.