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NMT2 Polyclonal antibody

NMT2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15720-1-AP

Print datasheet

Synonyms

Peptide N-myristoyltransferase 2, N myristoyltransferase 2, Myristoyl-CoA:protein N-myristoyltransferase 2, Glycylpeptide N-tetradecanoyltransferase 2, EC:2.3.1.97

验证数据展示

  • WB analysis using 15720-1-AP

    WB analysis using 15720-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15720-1-AP (NMT2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 15720-1-AP

    IHC staining of mouse testis using 15720-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15720-1-AP (NMT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U2OS using 15720-1-AP

    IF Staining of U2OS using 15720-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using NMT2 antibody (15720-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inHCT 116 cells, SKOV-3 cells, NIH/3T3 cells, rat testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15720-1-AP targets NMT2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen NMT2 fusion protein Ag8438 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name N-myristoyltransferase 2
Synonyms Peptide N-myristoyltransferase 2, N myristoyltransferase 2, Myristoyl-CoA:protein N-myristoyltransferase 2, Glycylpeptide N-tetradecanoyltransferase 2, EC:2.3.1.97
Calculated Molecular Weight529aa,60 kDa; 498aa,57 kDa
GenBank Accession NumberBC006376
Gene Symbol NMT2
Gene ID (NCBI) 9397
RRIDAB_3669219
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60551
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NMT2 antibody 15720-1-APDownload protocol
IHC protocol for NMT2 antibody 15720-1-APDownload protocol
IF protocol for NMT2 antibody 15720-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols