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验证数据展示

  • WB analysis using 15720-1-AP

    WB analysis using 15720-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15720-1-AP (NMT2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 15720-1-AP

    IHC staining of mouse testis using 15720-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15720-1-AP (NMT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U2OS using 15720-1-AP

    IF Staining of U2OS using 15720-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using NMT2 antibody (15720-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

NMT2 Polyclonal antibody

NMT2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15720-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Peptide N-myristoyltransferase 2, N myristoyltransferase 2, Myristoyl-CoA:protein N-myristoyltransferase 2, Glycylpeptide N-tetradecanoyltransferase 2, EC:2.3.1.97

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHCT 116 cells, SKOV-3 cells, NIH/3T3 cells, rat testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15720-1-AP targets NMT2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 NMT2 fusion protein Ag8438 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 N-myristoyltransferase 2
别名 Peptide N-myristoyltransferase 2, N myristoyltransferase 2, Myristoyl-CoA:protein N-myristoyltransferase 2, Glycylpeptide N-tetradecanoyltransferase 2, EC:2.3.1.97
计算分子量529aa,60 kDa; 498aa,57 kDa
GenBank蛋白编号BC006376
基因名称 NMT2
Gene ID (NCBI) 9397
RRIDAB_3669219
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO60551
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NMT2 antibody 15720-1-APDownload protocol
IHC protocol for NMT2 antibody 15720-1-APDownload protocol
IF protocol for NMT2 antibody 15720-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols