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验证数据展示

  • IHC staining of mouse brain using 14619-1-AP

    IHC staining of mouse brain using 14619-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14619-1-AP (NMUR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of human colon using 14619-1-AP

    WB analysis of human colon using 14619-1-AP

    human colon tissue were subjected to SDS PAGE followed by western blot with 14619-1-AP (NMUR1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 14619-1-AP

    IHC staining of mouse brain using 14619-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14619-1-AP (NMUR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NMUR1 Polyclonal antibody

NMUR1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14619-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

FM 3, FM3, G protein coupled receptor 66, GPC R, GPR66, neuromedin U receptor 1, NMUR1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman colon tissue, human stomach tissue, mouse pancreas tissue
Positive IHC detected inmouse brain tissue, human colon tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14619-1-AP targets NMUR1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 NMUR1 fusion protein Ag6235 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 neuromedin U receptor 1
别名 FM 3, FM3, G protein coupled receptor 66, GPC R, GPR66, neuromedin U receptor 1, NMUR1
计算分子量 47 kDa
观测分子量 47 kDa
GenBank蛋白编号BC036543
基因名称 NMUR1
Gene ID (NCBI) 10316
RRIDAB_2267310
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HB89
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Neuromedin-U (NMU) is a widely expressed peptide with the highest abundance in the central nervous system (CNS) and intestinal tract of nearly all vertebrate species. NMU mediates several physiological functions via its two cognate receptors, NMUR1 and NMUR2 (PMID:37102164). NMUR1(NmU receptor 1) has the highest expression in peripheral tissues, particularly the GI tract, pancreas, uterus, and testes but has also been reported in peripheral nerve terminals (PMID:10783389).

实验方案

Product Specific Protocols
WB protocol for NMUR1 antibody 14619-1-APDownload protocol
IHC protocol for NMUR1 antibody 14619-1-APDownload protocol
FC protocol for NMUR1 antibody 14619-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols