验证数据展示
经过测试的应用
| Positive WB detected in | human adrenal gland tissue, human heart tissue, human liver tissue |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13442-2-AP targets NNT in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | NNT fusion protein Ag4259 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | nicotinamide nucleotide transhydrogenase |
| 别名 | EC:7.1.1.1 |
| 计算分子量 | 1085 aa, 114 kDa |
| 观测分子量 | 114 kDa |
| GenBank蛋白编号 | BC032370 |
| 基因名称 | NNT |
| Gene ID (NCBI) | 23530 |
| RRID | AB_2153596 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q13423 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NNT(nicotinamide nucleotide transhydrogenase) is a transmembrane protein and functions as a proton pumping transhydrogenase. The protein is present in both prokaryotes and eukaryotes and is located in the inner membrane of mitochondria. In prokaryotic cells, the enzyme is composed of α and βsubunits of 54 and 48 kDa, respectively. In eukaryotic cells, the enzyme is usually composed of a single peptide of 110 kDa. Although NNT catalyzes the interconversion of NADH and NADPH, the forward reaction using the reducing power of NADH to regenerate NADPH would be favored under conditions of oxidative stress(PMID:16497723). It can exsit as a homodimer(PMID:21882037).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NNT antibody 13442-2-AP | Download protocol |
| IHC protocol for NNT antibody 13442-2-AP | Download protocol |
| IF protocol for NNT antibody 13442-2-AP | Download protocol |
| IP protocol for NNT antibody 13442-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Redox Biol Nicotinamide nucleotide transhydrogenase-mediated redox homeostasis promotes tumor growth and metastasis in gastric cancer.
| ||
Front Cell Dev Biol Dynamic Mitochondrial Proteome Under Polyamines Treatment in Cardiac Aging. | ||
Sci Rep Targeted Metabolomics Reveals Abnormal Hepatic Energy Metabolism by Depletion of β-Carotene Oxygenase 2 in Mice. | ||
Mol Nutr Food Res Lack of β, β-carotene -9', 10'-oxygenase 2 leads to hepatic mitochondrial dysfunction and cellular oxidative stress in mice. | ||
Am J Med Genet A Nicotinamide nucleotide transhydrogenase mutation analysis in Chinese patients with thyroid dysgenesis. |
