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NO66/C14orf169 Polyclonal antibody

NO66/C14orf169 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 22842-1-AP

Print datasheet

Synonyms

C14orf169, hsNO66, MAPJD, NO66, NO66/C14orf169, Nucleolar protein 66

验证数据展示

  • WB analysis using 22842-1-AP

    WB analysis using 22842-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22842-1-AP (NO66/C14orf169 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 22842-1-AP

    IP experiment of Jurkat using 22842-1-AP

    IP result of anti-NO66/C14orf169 (IP:22842-1-AP, 4ug; Detection:22842-1-AP 1:500) with Jurkat cells lysate 4000ug.

经过测试的应用

Positive WB detected inA431 cells, Jurkat cells, NCCIT cells
Positive IP detected inJurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 22842-1-AP is NO66/C14orf169 Fusion Protein expressed in E. coli.

Tested Applications WB, IP, ELISA Application Description
Tested Reactivity human
Immunogen NO66/C14orf169 fusion protein Ag18721 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 14 open reading frame 169
Synonyms C14orf169, hsNO66, MAPJD, NO66, NO66/C14orf169, Nucleolar protein 66
Calculated Molecular Weight 641 aa, 71 kDa
Observed Molecular Weight 71 kDa
GenBank Accession NumberBC011350
Gene Symbol NO66
Gene ID (NCBI) 79697
RRIDAB_2879175
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ9H6W3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NO66, also known as C14orf169 and MAPJD, is a JmjC domain-containing oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase. NO66 is highly conserved during evolution and shows a dual localization pattern, present in both the nucleoplasm and nucleolus (PMID: 14742713). It has been reported that NO66 participates in regulation of Osx transcriptional activity and plays an important role in osteoblast differentiation through interaction with Osx (PMID: 19927124; 23620590). Through interaction with MYC protein, NO66 plays a role in pulmonary carcinogenesis (PMID: 17308053).

实验方案

Product Specific Protocols
WB protocol for NO66/C14orf169 antibody 22842-1-APDownload protocol
IP protocol for NO66/C14orf169 antibody 22842-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols