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验证数据展示

  • WB analysis using 22842-1-AP

    WB analysis using 22842-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22842-1-AP (NO66/C14orf169 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 22842-1-AP

    IP experiment of Jurkat using 22842-1-AP

    IP result of anti-NO66/C14orf169 (IP:22842-1-AP, 4ug; Detection:22842-1-AP 1:500) with Jurkat cells lysate 4000ug.

NO66/C14orf169 Polyclonal antibody

NO66/C14orf169 Polyclonal Antibody for WB, IP, ELISA
Cat No. 22842-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, ELISA

别名

C14orf169, hsNO66, MAPJD, NO66, NO66/C14orf169, Nucleolar protein 66

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, Jurkat cells, NCCIT cells
Positive IP detected inJurkat cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22842-1-AP targets NO66/C14orf169 in WB, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human
免疫原 NO66/C14orf169 fusion protein Ag18721 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 14 open reading frame 169
别名 C14orf169, hsNO66, MAPJD, NO66, NO66/C14orf169, Nucleolar protein 66
计算分子量 641 aa, 71 kDa
观测分子量 71 kDa
GenBank蛋白编号BC011350
基因名称 NO66
Gene ID (NCBI) 79697
RRIDAB_2879175
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ9H6W3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NO66, also known as C14orf169 and MAPJD, is a JmjC domain-containing oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase. NO66 is highly conserved during evolution and shows a dual localization pattern, present in both the nucleoplasm and nucleolus (PMID: 14742713). It has been reported that NO66 participates in regulation of Osx transcriptional activity and plays an important role in osteoblast differentiation through interaction with Osx (PMID: 19927124; 23620590). Through interaction with MYC protein, NO66 plays a role in pulmonary carcinogenesis (PMID: 17308053).

实验方案

Product Specific Protocols
WB protocol for NO66/C14orf169 antibody 22842-1-APDownload protocol
IP protocol for NO66/C14orf169 antibody 22842-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols