验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, SGC-7901 cells, HepG2 cells, K-562 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | mouse brain tissue, human colon cancer tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| WB | See 6 publications below |
| IHC | See 1 publications below |
产品信息
10091-2-AP targets NOB1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | NOB1 fusion protein Ag0141 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | NIN1/RPN12 binding protein 1 homolog (S. cerevisiae) |
| 别名 | NOB1P, MSTP158, MST158, EC:3.1.-.-, ART4 |
| 计算分子量 | 47 kDa |
| 观测分子量 | 47-50 kDa |
| GenBank蛋白编号 | BC000050 |
| 基因名称 | NOB1 |
| Gene ID (NCBI) | 28987 |
| RRID | AB_2298640 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9ULX3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NOB1 was first identified in yeast as an essential gene encoding the Nin one binding protein, which is involved in pre-rRNA processing. In a late cytoplasmic processing step, Nob1 cleaves a 20S rRNA intermediate at cleavage site D to produce the mature 18S rRNA. In addition, NOB1 is a crucial molecule in the maturation of the 20S proteasome and protein degradation. It serves as a chaperone to join the 20S proteasome with the 19S regulatory particle in the nucleus and facilitates the maturation of the 20S proteasome. Recently NOB1 has been reported to be overexpressed in several types of cancer, suggesting its involvement in the tumorigenesis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NOB1 antibody 10091-2-AP | Download protocol |
| IHC protocol for NOB1 antibody 10091-2-AP | Download protocol |
| IF protocol for NOB1 antibody 10091-2-AP | Download protocol |
| IP protocol for NOB1 antibody 10091-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Molecular basis for disassembly of an importin:ribosomal protein complex by the escortin Tsr2. | ||
Elife A RanGTP-independent mechanism allows ribosomal protein nuclear import for ribosome assembly. | ||
Elife Prefabrication of a ribosomal protein subcomplex essential for eukaryotic ribosome formation. | ||
World J Gastroenterol NOB1 is essential for the survival of RKO colorectal cancer cells.
| ||
Oncol Rep NOB1 silencing inhibits the growth and metastasis of laryngeal cancer cells through the regulation of JNK signaling pathway. | ||
Oncol Lett Effects of NOB1 on the pathogenesis of osteosarcoma and its expression on the chemosensitivity to cisplatin.
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