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验证数据展示

  • WB analysis of human liver using 15297-1-AP

    WB analysis of human liver using 15297-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 15297-1-AP (RPH3AL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 15297-1-AP

    IP experiment of HepG2 using 15297-1-AP

    IP result of anti-RPH3AL (IP:15297-1-AP, 4ug; Detection:15297-1-AP 1:500) with HepG2 cells lysate 2400ug.

RPH3AL Polyclonal antibody

RPH3AL Polyclonal Antibody for WB, IP, ELISA
Cat No. 15297-1-AP

Print datasheet

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Synonyms

No C2 domains protein, NOC2, Rab effector Noc2, Rabphilin 3A like protein, RPH3AL

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inhuman liver tissue, L02 cells
Positive IP detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

15297-1-AP targets RPH3AL in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen RPH3AL fusion protein Ag7552 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name rabphilin 3A-like (without C2 domains)
Synonyms No C2 domains protein, NOC2, Rab effector Noc2, Rabphilin 3A like protein, RPH3AL
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC005153
Gene Symbol RPH3AL
Gene ID (NCBI) 9501
RRIDAB_2181270
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UNE2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Rabphilin-3A-like (RPH3AL), also known as Noc2, is a Rab effector protein with a molecular weight of 38 kDa. It is expressed abundantly in endocrine cells but at low levels in exocrine tissues, playing a direct regulatory role in calcium-ion-dependent exocytosis in both endocrine and exocrine cells, and also having a key role in INS secretion by pancreatic cells. RPH3AL functions as a potential RAB3B effector protein in epithelial cells, and RPH3AL gene is likely a tumor suppressor.

实验方案

Product Specific Protocols
WB protocol for RPH3AL antibody 15297-1-APDownload protocol
IP protocol for RPH3AL antibody 15297-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Cell Sci

Rab2a and Rab27a cooperatively regulate transition from granule maturation to exocytosis through the dual effector Noc2.

Authors - Kohichi Matsunaga
View Article
humanWB

Clin Chim Acta

Detection of autoantibodies against Rabphilin-3A-like protein as a potential biomarker in patient's sera of colorectal cancer.

Authors - Jinn-Shiun Chen
View Article